Stabilization of recombinant interferon-alpha by pegylation for encapsulation in PLGA microspheres |
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| Authors: | Diwan Manish Park Tae Gwan |
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| Affiliation: | Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 305-701, Daejeon, South Korea. |
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| Abstract: | Interferon-alpha (IFN) was pegylated and encapsulated in biodegradable microspheres to achieve a long-acting formulation. IFN was pegylated with methoxy-polyethylene glycol (mPEG, MW 2000 or 5000). The conjugation procedures were optimized in terms of concentration of the reactants and the pH condition of the medium. The conjugates (IFN-mPEG(2000) and IFN-mPEG(5000)) were characterized using SDS-PAGE, size-exclusion-HPLC (SE-HPLC) and matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectroscopy. The optimized IFN-mPEG conjugates consisted of mono- and multi-pegylated derivatives along with a small amount of native IFN (
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