中国HCV/HIV合并感染者T淋巴细胞表面CD81、CXCR3表达的研究

目的探讨T淋巴细胞表面受体CD81、CXCR3，在丙肝病毒（HCV）单纯感染，艾滋病病毒（HIV）单纯感染和HCV／HIV合并感染过程中的表达及意义。方法采用流式细胞术，检测HCV感染组（n=21），HIV感染组（n=14），HCV／HIV感染组（n=28）及正常对照组（n=30）人外周血CD4^＋T淋巴细胞和CD8^＋T淋巴细胞表面CD81、CXCR3的表达。结果HIV感染组及HCV／HIV合并感染组CD4^＋T细胞表面CD81、CXCR3表达显著降低（P〈0．001），CD8^＋T细胞表面CD81、CXCR3表达显著升高（P〈0．001）；HCV感染组CD4^＋及CD8^＋T细胞表面CXCR3表达轻度升高但差异不显著，CD81在CD4^＋T细胞表面轻度升高而在CD8^＋T细胞表面明显升高（P〈0．01）。结论中国HCV／HIV合并感染患者外周血T淋巴细胞表面受体CD81、CXCR3，在CD4^＋T细胞表面表达降低，而在CD8^＋T细胞表面表达升高。

Study on surface expression of CD81 and CXCR3 on T lymphocytes from patients with HCV/HIV co-infection in China

Objective To investigate expression and significance of CD81,CXCR3 in HCV mono-infection,HIV mono-infection and HCV/HIV co-infection.Methods We detected the surface expression of CD81 and CXCR3 on CD4~+T cells and CD8~+T cells from PBMC isolated from HCV infection(n=21),HIV infection(n=14),HCV/HIV co-infection(n=28) and normal control(n=30) by flow cytometry.Results The expression of CD81 and CXCR3 on CD4~+T cells decreased significantly(P<0.001),whereas it significantly increased on CD8~+T cells in HIV infection and HCV/HIV co-infection group(P<0.001).In HCV infection group,the expression of CXCR3 on CD4~+T cells as well as CD8~+T cells increased but not significantly,and expression of CD81 on CD4~+T cells slightly increased but significantly increased on CD8~+T cells(P<0.01).Conclusion The surface expression of CD81 and CXCR3 on CD4~+ T lymphocytes decreased,whereas it increased on CD8~+T lymphocytes from patients with HCV/HIV co-infection in China.