| 二硫苏糖醇代替2巯基乙醇在IgG抗A(B)测定中的应用 |
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| 引用本文： | 王雪华,林少微,刘秋云,黄让云,许慕明. 二硫苏糖醇代替2巯基乙醇在IgG抗A(B)测定中的应用[J]. 实用医技杂志, 2005, 12(21): 3040-3041 |
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| 作者姓名： | 王雪华 林少微 刘秋云 黄让云 许慕明 |
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| 作者单位： | 1. 汕头大学医学院第一附属医院,广东,汕头,515041
2. 汕头大学医学院肿瘤医院,广东,汕头,515041 |
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| 摘 要： | 目的:评价用二硫苏糖醇代替2巯基乙醇灭活IgM抗体在IgG抗A(B)测定中的应用。方法:80份O型孕妇血清分别经2巯基乙醇和二硫苏糖醇灭活处理,然后进行IgG抗A(B)效价测定。结果:经2巯基乙醇处理的阳性检出率44.7%;经二硫苏糖醇处理的阳性检出率45.9%。经χ2检验,两种方法的检测结果差异无显著性(χ2=0.024,P=0.052 4)。结论:用二硫苏糖醇代替2巯基乙醇灭活处理血清具有无恶臭味、反应快速等优点,可提高工作效率,并减少环境污染,值得推广。
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| 关 键 词： | 2巯基乙醇 二硫苏糖醇 ABO血型系统 新生儿溶血病 |
Application of Dithiothreitol Instead of 2-mercaptoethanol in Detection of Anti-type A(or B) IgG Antibody |
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| WANG Xue-hua,LIN Shao-wei,LIU Qiu-yun,et al. Application of Dithiothreitol Instead of 2-mercaptoethanol in Detection of Anti-type A(or B) IgG Antibody[J]. Journal of Practical Medical Techniques, 2005, 12(21): 3040-3041 |
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| Authors: | WANG Xue-hua LIN Shao-wei LIU Qiu-yun et al |
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| Affiliation: | WANG Xue-hua~1,LIN Shao-wei~1,LIU Qiu-yun~1,et al |
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| Abstract: | Objective to evaluate the application of dithiothreitol(DTT) instead of 2-Mercaptoethanol(2-me) in the detection of anti-type A(or B) IgG antibody.Methods Blood sera were obtained from 80 pregnant women whose blood type were O.All the blood sera were inactivated by DTT and 2-me respectively.And then the titer of anti-type A(or B) IgG antibody was mensurated.Results The positive rate of the blood sera which were treated by DTT was 45.9%,and it was 44.7% which were treated by 2-me.There was no significance between the two methods(χ~2=0.024,P=0.052 4).Conclusion DTT can be used to inactivated blood serum instead of 2-me.It has good qualities of no stink,quick reaction and efficient, etc.It can also reduce the environmental pollution.Therefore,we recommend the method to be used widely. |
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| Keywords: | 2-Mercaptoethanol Dithiothreitol ABO Blood-Group system Hemolytic disease of newborn |
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