| 当归对单侧输尿管梗阻大鼠肾组织中黏附分子ICAM-1表达的影响 |
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| 引用本文： | 黄岩杰,马宁宁,杨晓青,刘霞,张建,郑春燕,任献青,翟文生,丁樱,郑建民. 当归对单侧输尿管梗阻大鼠肾组织中黏附分子ICAM-1表达的影响[J]. 中华中医药杂志, 2011, 0(1): 167-170 |
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| 作者姓名： | 黄岩杰 马宁宁 杨晓青 刘霞 张建 郑春燕 任献青 翟文生 丁樱 郑建民 |
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| 作者单位： | 河南中医学院第一附属医院儿科医院;河南中医学院;河南省中医药研究院;河南中医学院第三附属医院; |
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| 基金项目： | 河南省教育厅自然科学基金项目(No.2006360020)~~ |
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| 摘 要： | 目的:观察当归对单侧输尿管梗阻(UUO)大鼠肾小管间质纤维化过程中黏附分子P-选择素(P-selectin)和细胞间黏附分子-1(ICAM-1)、转化生长因子-β1(TGF-β1)等细胞因子的表达和CD68单核/巨噬细胞浸润的影响。方法:采用大鼠模型,52只SD大鼠分为:假手术组、UUO组、当归组。术后第7d和第14d留取标本处死大鼠,用免疫组化方法检测肾组织内P-selectin、ICAM-1、CD68、TGF-β1的表达。结果:与假手术组相比,UUO组大鼠第7d和第14d肾组织内ICAM-1、P-selectin、CD68、TGF-β1的表达均显著上调(P<0.05)。与UUO组相比,当归组两个时间段肾小管间质病变减轻,且肾组织内ICAM-1、CD68、TGF-β1的表达受到显著抑制(P<0.05)。结论:当归可能是通过抑制ICAM-1的表达和炎细胞的组织浸润,从而降低了肾小管间质TGF-β1的水平和纤维化的进展。
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| 关 键 词： | 肾小管间质纤维化 当归 黏附分子P-选择素 细胞间黏附分子-1 CD68单核/巨噬细胞 转化生长因子-β1 |
Effect of Angelica on renal ICAM-1 expression in unilateral ureteral obstruction rats |
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| HUANG Yan-jie,MA Ning-ning,YANG Xiao-qing,LIU Xia,ZHANG Jian,ZHENG Chun-yan,REN Xian-qing,ZHAI Wen-sheng. Effect of Angelica on renal ICAM-1 expression in unilateral ureteral obstruction rats[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2011, 0(1): 167-170 |
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| Authors: | HUANG Yan-jie MA Ning-ning YANG Xiao-qing LIU Xia ZHANG Jian ZHENG Chun-yan REN Xian-qing ZHAI Wen-sheng |
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| Affiliation: | HUANG Yan-jie1,MA Ning-ning2,YANG Xiao-qing1,LIU Xia1,ZHANG Jian1,ZHENG Chun-yan1,REN Xian-qing1,ZHAI Wen-sheng1(Director'DING Ying1,ZHENG Jian-min1)(1Department of Pediatrics,the First Affiliated Hospital of Henan University of TCM,Zhengzhou 450008,China,2Henan University of TCM,Zhengzhou 450000,3Chinese Medicine Research Institute of Henan Province,ZhengZhou 450000,4The Third Affiliated Hospital of Henan University of TCM,China) |
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| Abstract: | Objective: To investigatethe effect of angelica on the expression of P-selectin,Intercellular Cell Adhesion Molecule-1(ICAM-1),and Transforming Growth Factor-1(TGF-β1),and the infiltration of macrophage in kidney of UUO rats.Methods: the unilateral ureteral obstruction(UUO) rat model was studied in this experiment.52 male SD rats were randomly divided into: ① sham group;② UUO model group;③Angelica group.Half rats in each group were killed respectively on day 7 and day 14.Renal tissue P-selectin,ICAM-1,CD68,TGF-β1 expression and localization were detected by immmunohistochemical analysis.Results: Compared with the sham group,the levels of renal tissue ICAM-1,P-selectin,CD68,TGF-β1 expression were significantly upregulated in UUO model group on day 7 and day 14(P<0.05).Angelica decreased tubulointerstitial injury score from(5.56±0.56) to(4.42±0.89),and the renal interstitial inflammatory cell count,and suppressed significantly the levels of renal tissue ICAM-1,CD68,TGF-β1 expression on day 7 and day 14,compared with the UUO model group(P<0.05).Conclusion: Angelica meliorates development of tubulointerstitial fibrosis via decreased ICAM-1 expression and inflammatory cell infiltration in UUO rats. |
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| Keywords: | Tubulointerstitial fibrosis Angelica P-selectin ICAM-1 CD68 TGF-β1 |
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