| 附子理中丸对脾阳虚证大鼠骨骼肌肌球蛋白ATP酶活性的影响 |
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| 引用本文： | 张文通,唐汉庆,卢阿娜,王晓怡,杨艳,罗静,杨美娟,李国璋,蒋玉凤,蔡大勇.附子理中丸对脾阳虚证大鼠骨骼肌肌球蛋白ATP酶活性的影响[J].中华中医药杂志,2011(3):490-494. |
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| 作者姓名： | 张文通 唐汉庆 卢阿娜 王晓怡 杨艳 罗静 杨美娟 李国璋 蒋玉凤 蔡大勇 |
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| 作者单位： | 中国医学科学院药用植物研究所;北京市回民医院;北京中医药大学基础医学院; |
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| 基金项目： | 国家重大基础研究(No.G2000056905); 国家自然科学基金(No.30873464); 教育部重点研究(No.108019)~~ |
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| 摘 要： | 目的:观察附子理中丸对脾阳虚证大鼠骨骼肌肌球蛋白ATP酶(M-ATP酶)活性的影响。方法:18窝新生大鼠,同窝9只匹配、体质量均衡随机分入9组;前5组不切除甲状腺为获得性脾阳虚;后4组切除甲状腺为发育性脾阳虚。第1d切除部分甲状腺,第42d切除肩胛骨间棕色脂肪,第56d开始高脂饲养,第70d开始用药防治,第98d取左侧腓肠肌,冰冻切片,M-ATP酶染色,形态计量单位体积相对总活性,t检验比较组间差别。结果:获得性脾阳虚中,模型组M-ATP酶活性比对照组升高26.10%(P<0.01),高脂组比模型组降低10.40%(P<0.01),凉治组比高脂组降低21.39%(P<0.01),温治组比高脂组升高34.89%(P<0.01)。发育性脾阳虚中,模型组M-ATP酶活性比对照组升高101.56%(P<0.01),高脂组比模型组降低19.98%(P<0.01),凉治组比高脂组升高8.01%(P<0.01),温治组比高脂组降低18.65%(P<0.01)。与获得性脾阳虚比较,性脾阳虚模型组、高脂组、凉治组和温治组分别升高59.85%(P<0.01)、42.76%(P<0.01)、96.14%(P<0.01)和18.29%(P<0.01)。结论:附子理中丸回调脾阳虚大鼠骨骼肌M-ATP酶活性,对发育性脾阳虚大鼠的作用效能大于获得性脾阳虚的作用效能。
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| 关 键 词： | 附子理中丸 脾阳虚证 大鼠 骨骼肌 M-ATP酶活性 |
Fuzi Lizhong Pills regulating myosin ATPase activity of skeletal muscle in rats with spleen yang deficient syndrome |
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| ZHANG Wen-tong,TANG Han-qing,LU A-na,WANG Xiao-yi,YANG Yan,LOU Jing,YANG Mei-juan,LI Guo-zhang,JIANG Yu-feng,CAI Da-yong.Fuzi Lizhong Pills regulating myosin ATPase activity of skeletal muscle in rats with spleen yang deficient syndrome[J].China Journal of Traditional Chinese Medicine and Pharmacy,2011(3):490-494. |
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| Authors: | ZHANG Wen-tong TANG Han-qing LU A-na WANG Xiao-yi YANG Yan LOU Jing YANG Mei-juan LI Guo-zhang JIANG Yu-feng CAI Da-yong |
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| Institution: | ZHANG Wen-tong1,2,TANG Han-qing1,3,LU A-na1,WANG Xiao-yi3,YANG Yan3,LOU Jing3,YANG Mei-juan3,LI Guo-zhang3,JIANG Yu-feng3,CAI Da-yong1,3(1Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China,2Beijing Hui Hospital,Beijing 100054,3College of Preclinical Medicine,Beijing University of Chinese Medicine,Beijing 100029,China) |
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| Abstract: | Objective: To observe the effect of Fuzi Lizhong Pills on the myosin ATPase(M-ATPase) activity of the skeletal muscle in rats with spleen yang deficient syndrome.Methods: Eighteen litters of rats were used,and nine littermates in each litter were bred sequentially and randomized into 9 groups.Five groups were not carried out thyroidectomy,the other four groups did.Thyroidectomy in hypothyroidism littermates was carried out with 131iodine to destroy partly thyroidal tissue at d1 after birth,the interscapular... |
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| Keywords: | Fuzi Lizhong Pills Spleen yang defi cient syndrome Rats Skeletal muscle Myosin ATPase activity |
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