| 两种老年失眠证候大鼠模型的建立和评价 |
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| 引用本文： | 游秋云,王平,吴丽丽,田代志,黄攀攀,胡慧.两种老年失眠证候大鼠模型的建立和评价[J].中华中医药杂志,2011(1):123-126. |
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| 作者姓名： | 游秋云 王平 吴丽丽 田代志 黄攀攀 胡慧 |
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| 作者单位： | 湖北中医药大学药学院;湖北中医药大学中药资源与中药复方省部共建教育部重点实验室;湖北武钢总医院; |
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| 基金项目： | 国家自然科学基金2010年面上项目(No.81072849); 武汉市科技攻关计划项目(No.200960323121)~~ |
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| 摘 要： | 目的:建立并评价2种复合型老年失眠证候大鼠模型。方法:分别采用腹腔注射D-半乳糖+夹尾刺激+睡眠剥夺,腹腔注射D-半乳糖+环磷酰胺及氢化可的松+睡眠剥夺制作肝郁、血亏阴虚型老年失眠证候大鼠模型,观测大鼠体征、脑部自由基水平、血浆6-keto-PGF1α、TXB2及血象含量。结果:与空白对照组、单因素模型组比较,2种模型组的自由基水平均显著增高(P<0.05,P<0.01);与其他组比较,老年肝郁失眠证候模型大鼠TXB2含量显著降低,6-keto-PGF1α含量及6-keto-PGF1α/TXB2比值均显著升高(P<0.01),而老年血亏阴虚失眠证候模型大鼠全血血象显著降低(P<0.01)。结论:老年肝郁及血亏阴虚失眠证候大鼠模型均具有临床证候病理特征,是可行、可靠的复合型动物模型。
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| 关 键 词： | 大鼠模型 睡眠剥夺 老年 肝郁 血亏阴虚 |
Establishment and evaluation of the two kinds of aging rat pathogeny models of insomnia |
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| YOU Qiu-yun,WANG Ping,WU Li-li,TIAN Dai-zhi,HUANG Pan-pan,HU Hui.Establishment and evaluation of the two kinds of aging rat pathogeny models of insomnia[J].China Journal of Traditional Chinese Medicine and Pharmacy,2011(1):123-126. |
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| Authors: | YOU Qiu-yun WANG Ping WU Li-li TIAN Dai-zhi HUANG Pan-pan HU Hui |
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| Abstract: | Objective: To establish and evaluate the two kinds of aging rat pathogeny models of insomnia.Methods: The insomnia rats model with the pathogeny of aging Liver-qi stagnationwas induced by D-galactose neck back subcutaneously,the stimulation of the rats tail leading to Liver-qi stagnation,sleep deprivation using modified multiple platform method(MMPM).The other insomnia rats model with the pathogeny of aging Blood-deficiency and Yin was induced by D-galactose neck back subcutaneously,intraperitoneal injectio... |
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| Keywords: | Rats model Sleep deprivation(SD) Aging Liver-qi stagnation Blood-deficiency and yin |
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