维生素B6注射液对小鼠小肠蠕动的影响及其机制

目的研究维生素(Vit)B6注射液对小鼠小肠蠕动的影响及其机制。方法小鼠随机分9g/L盐水(0.1mL/10g腹腔注射)对照组、高剂量VitB6组(5mg/10g腹腔注射)、低剂量VitB6组(0.5mg/10g腹腔注射)及氯化钙(1mg/10g腹腔注射)、新斯的明(0.0015mg/10g腹腔注射)、硫酸阿托品(0.005mg/10g腹腔注射)为模型的对照组,并在此基础上分别合用高和低剂量VitB6注射液为实验组进行小肠墨汁推进实验。各组给药30min后予墨汁(0.1mL/20g)灌胃,20min后颈椎脱臼处死,剖腹测量墨汁推进和小肠总长度,计算墨汁推进率。结果高剂量VitB6能明显抑制正常肠蠕动(P<0.01),高、低剂量VitB6均能明显拮抗氯化钙作用下肠蠕动增强(P<0.01),高剂量VitB6能协同加强硫酸阿托品肠蠕动抑制作用(P<0.01),高、低剂量VitB6对甲硫新斯的明致肠蠕动增强有一定拮抗作用,但无统计学意义(Pa>0.05)。结论VitB6注射液对小鼠小肠亢进有拮抗作用,以高剂量作用明显,为治疗肠痉挛提供理论根据。

Effects of Vitamin B6 Injection on Small Intestinal Peristalsis in Mice and Its Mechanisms

Objective To study the effects of vitamin B_6(VitB_6) injection on small intestinal peristalsis in mice and its mechanisms.Methods The mice were divided into 12 groups:calcium chloride injection group(1 mg/10 g),neostigmine methylsulfate injection group(0.001 5 mg/10 g),atropine sulfate injection group(0.005 mg/10 g),their combination with VitB_6 injection and high/low dose treated groups,high dose VitB_6 injection group(5 mg/10 g),low dose VitB_6 injection group(0.5 mg/10 g) and physiologic saline group(0.1 mL/ 10 g ).After administration 30 minutes,mice were intragastric administration Indian ink(0.1 mL/g),and they were luxated and put to death 20 minutes later.The mice belly were cut open,the length of intestine and distance of Indian ink that had moved were measured,and then the ink progradation rate were calculated.Results Compared with control group,the high dose VitB_6 injection could inhibit normal intestinal peristalsis of mice markedly(P<0.01).Both of high dose and low dose VitB_6 injection could inhibit the excited action of calcium chloride significantly( P_a< 0.01),and high dose VitB_6 injection could enhance the inhibited action of atropine(P<0.01).Both of high dose and low dose VitB_6 injection had antagonistic effect on the excited action of proserine methylsulfate,but it was not statistically significant(P_a>0.05).Conclusions VitB_6 injection can inhibit hyperanakinesia of small intestine in mice,especially high dose.And this will be provided as theory foundation on enterospasm treatment.