Numb蛋白在上皮性卵巢癌中的表达及其与CD117、CD133、ALDH1的关系

目的 探讨膜相关蛋白Numb在上皮性卵巢癌中的表达及其与卵巢癌干细胞标志物CD117、CD133、乙醛脱氢酶1（ALDH1）的关系。方法 选择四川大学华西第二医院妇科住院的136例卵巢肿瘤患者及无卵巢肿瘤患者22例为研究对象，根据摘除卵巢的病理组织学检查结果，将其分为上皮性卵巢癌组（n=92）、交界性卵巢肿瘤组（n=23）、良性卵巢肿瘤组（n=21）及正常卵巢组（n=22）。采用免疫组化SP法检测患者卵巢组织中Numb、CD117、CD133及ALDH1蛋白的表达水平，统计分析几种蛋白的表达差异及相关性。结果 ①Numb蛋白在上皮性卵巢癌组阳性表达率高于良性卵巢肿瘤组及正常卵巢组，交界性卵巢肿瘤组Numb蛋白阳性表达率高于正常卵巢组，且差异均有统计学意义（P<0.05）。②Numb蛋白阳性表达率在上皮性卵巢癌患者中FIGO分期为Ⅰ～Ⅱ期患者低于Ⅲ～Ⅳ期患者，无淋巴结转移患者低于有淋巴结转移患者，且阳性表达率差异均有统计学意义（P<0.05）；而不同年龄、组织病理学类型、病理分级及残留灶大小之间比较，差异则无统计学意义(P>0.05)。③上皮性卵巢癌患者卵巢组织中Numb蛋白与CD117及CD133阳性表达无相关性（r=0.116，P=0.261;r=0.083，P=0.425）；而Numb蛋白阳性表达与ALDH1表达呈正相关关系（r=0.296，P=0.003）。结论 Numb蛋白的表达与上皮性卵巢癌的发生、侵袭和转移有关，与卵巢癌干细胞标志物ALDH1表达呈正相关，有可能影响患者的预后。

Expression Level of Membrane-associated Proteins Numb in Epithelial Ovarian Carcinoma and Its Relationship

Objective To explore the expression level of membrane-associated protein Numb in epithelial ovarian carcinoma and its relationship with ovarian cancer stem cell markers CD117, CD133, acetaldehyde dehydrogenase 1(ALDH1). Methods A total of 136 patients who had ovarian tumors and 22 patients who had not ovarian tumors in Department of Gynaecology and Obstetrics, West China Second University Hospital, Sichuan University were chosen as the study subjects. According to the histopathologic examination results, they were divided into epithelial ovarian carcinoma group (n=92), ovarian borderline tumor group (n=23), ovarian benign tumor group (n=21) and normal ovary group (n=22). Expression levels of Numb protein, CD117, CD133 and ALDH1 in ovarian tissue were detected by immunohistochemical SP method and these several kinds of protein expression differences and correlation statistical analysis were performend. Results ① The positive expression rate of Numb protein in epithelial ovarian carcinoma group was higher than that in benign tumor or normal ovary group, also Numb protein positive expression rate in ovarian borderline tumor group was higher than that in normal ovary group, and the differences were statistically significant (P<0.05). ② Numb protein positive expression rate in ovarian tissue in patients with epithelial ovarian carcinoma FIGO stageⅠ-Ⅱ was lower than that in stage Ⅲ-Ⅳ, also the same in no lymph nodes metastasis compared with lymph nodes invasion, and the differences of positive expression rate were statistically significant (P<0.05). While there were no significant differences among different age, histopathological types, pathological grades and residual tumor size (P>0.05). ③ There was no correlation between Numb protein and CD117 and CD133 positive expression rate in epithelial ovarian carcinoma tissue 〔correlation coefficient (r)=0.116, P=0.261; r=0.083, P=0.425〕. However, the positive expression rate of Numb protein and ALDH1 was positively correlated (r=0.296, P=0.261). Conclusion The expression of Numb protein may involve in the occurrence, invasion and mechanisms of ovarian cancer, positively correlated with ovarian cancer stem cell markers ALDH1, and may affect the prognosis of patients.