| N-乙酰半胱氨酸活性炭缓释微囊对大鼠肝纤维化中细胞内成纤信号分子表达的影响 |
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| 引用本文： | 缪樑斌,包剑锋,刘寿荣,方红英,杨宗信,黄从想,潘熠健,高建青.N-乙酰半胱氨酸活性炭缓释微囊对大鼠肝纤维化中细胞内成纤信号分子表达的影响[J].中国现代应用药学,2016,33(11):1387-1392. |
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| 作者姓名： | 缪樑斌 包剑锋 刘寿荣 方红英 杨宗信 黄从想 潘熠健 高建青 |
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| 作者单位： | 1、浙江大学药学院；2、浙江中医药大学附属杭州西溪医院,浙江中医药大学附属杭州西溪医院,浙江中医药大学附属杭州西溪医院,浙江中医药大学附属杭州西溪医院,浙江中医药大学附属杭州西溪医院,浙江中医药大学附属杭州西溪医院,浙江中医药大学附属杭州西溪医院,浙江大学药学院 |
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| 基金项目： | 杭州市医药卫生科技计划项目 |
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| 摘 要： | 目的 研究口服乙酰半胱氨酸活性炭缓释微囊(ACNAC)在大鼠肝纤维化治疗中肝细胞内成纤信号分子表达的影响。方法 以明胶包裹吸附乙酰半胱氨酸(NAC)的载药活性炭制备得到ACNAC，以四氯化碳(CCl4)制备大鼠肝纤维化模型，ACNAC高、中、低剂量组(80，40，20 mg·kg?1·d?1，按照乙酰半胱氨酸计算)进行灌胃给药，NAC、水飞蓟宾为阳性对照药物。治疗8周后处死大鼠，通过大鼠肝功能指标、病理切片、免疫组织化学法评价ACNAC对胞内信号分子表达中的影响。结果 CCl4大鼠肝纤维化模型制备成功，其大鼠肝组织病理切片HE染色和网状纤维染色表明其肝纤维化程度为Ⅲ-Ⅳ期，免/疫组化显示胞内TGF-β1/Tβ-R1和Smad2/3大量表达。ACNAC、NAC和SM对肝纤维化大鼠血清ALT、AST及Tbil有显著的治疗作用，ACNAC高剂量组效果明显优于NAC和SM (p<0.05)。ACNAC各治疗组大鼠肝组织病理切片表明肝纤维化好转，免疫组化显示胞内TGF-β1/Tβ-R1和Smad2/3表达减少，ACNAC高剂量组表达具显著性差异(P<0.05)。结论 ACNAC对大鼠肝纤维化胞内成纤信号分子TGF-β1/Tβ-R1和Smad2/3有明显抑制作用，对抗大鼠肝纤维化的疗效明显，且较等剂量的NAC及对照药物水飞蓟宾有更显著的疗效。
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| 关 键 词： | N-乙酰半胱氨酸活性炭缓释微囊 肝纤维化 成纤信号分子 |
| 收稿时间： | 2016/3/22 0:00:00 |
| 修稿时间： | 2016/11/7 0:00:00 |
Effect of N-Acetylcysteine Activated Carbon Sustained-release Microcapsules on the Expression of Intracellular Fibroblast Signaling Molecules in Rat Liver Fibrosis |
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| MIAO Liangbin,BAO Jianfeng,LIU Shourong,FANG Hongying,YANG Zongxin,HUANG Congxiang,PAN Yijian and GAO Jianqing.Effect of N-Acetylcysteine Activated Carbon Sustained-release Microcapsules on the Expression of Intracellular Fibroblast Signaling Molecules in Rat Liver Fibrosis[J].The Chinese Journal of Modern Applied Pharmacy,2016,33(11):1387-1392. |
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| Authors: | MIAO Liangbin BAO Jianfeng LIU Shourong FANG Hongying YANG Zongxin HUANG Congxiang PAN Yijian and GAO Jianqing |
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| Institution: | College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China;Xixi Hospital of Hangzhou, Affiliated to Zhejiang Chinese Medicine University, Hangzhou 310023, China,Xixi Hospital of Hangzhou, Affiliated to Zhejiang Chinese Medicine University, Hangzhou 310023, China,Xixi Hospital of Hangzhou, Affiliated to Zhejiang Chinese Medicine University, Hangzhou 310023, China,Xixi Hospital of Hangzhou, Affiliated to Zhejiang Chinese Medicine University, Hangzhou 310023, China,Xixi Hospital of Hangzhou, Affiliated to Zhejiang Chinese Medicine University, Hangzhou 310023, China,Xixi Hospital of Hangzhou, Affiliated to Zhejiang Chinese Medicine University, Hangzhou 310023, China,Xixi Hospital of Hangzhou, Affiliated to Zhejiang Chinese Medicine University, Hangzhou 310023, China and College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China |
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| Abstract: | |
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| Keywords: | N-acetylcysteine activated carbon microcapsule (ACNAC) hepatic fibrosis Hepatic fibrosis fiber signal molecule |
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