补阳还五汤联合依达拉奉对脑缺血再灌注后神经细胞凋亡及Bcl-2和Bax表达的影响

目的 研究补阳还五汤联合依达拉奉对小鼠脑缺血再灌注损伤后神经细胞凋亡及凋亡相关蛋白表达的影响。方法 60 只小鼠随机分假手术组（sham），模型组（I/R），补阳还五汤组（BYHWD），依达拉奉组（ED），补阳还五汤+依达拉奉组（BYHWD+ED），每组再分2个亚组：1 d组和7 d 组。采用改良线栓法制作小鼠大脑中动脉缺血再灌注模型，分别给药。TUNEL法观察神经细胞凋亡率；进一步Western bolt检测大脑皮质缺血区bcl-2 和 bax的蛋白的定量表达。结果 补阳还五汤+依达拉奉组较其他组能更明显地降低神经细胞凋亡指数(P<0.01)，且较其他组可更明显地升高凋亡相关蛋白bcl-2的表达和下调bax的表达 (P<0．05)。结论 补阳还五汤与依达拉奉联用能降低脑缺血再灌注损伤后小鼠脑组织细胞中促凋亡蛋白bax的表达，增加具有神经元保护作用的抑凋亡蛋白bcl-2的表达，从而抑制神经细胞凋亡，加速神经功能的恢复，协同发挥脑保护作用。

Effects of Buyang Huanwu Decoction Combined with Edaravone on the Apoptosis of Neuron and Expression of Bcl-2 and Bax Following Cerebral Ischemia-reperfusion in Mice

OBJECTIVE To study the effects of Buyang Huanwu decoction(BYHWD) combined with edaravone(ED) on the apoptosis of neuron and expression of apoptosis related proteins following cerebral ischemia reperfusion in mice. METHODS Sixty mice were randomly divided into sham group, I/R group, BYHWD group, ED group, BYHWD+ED group. Each group were further randomly divided into 1 d and 7 d group. The middle cerebral artery ischemia/reperfusion model was established by the improved intraluminal filament technique in mice. TUNEL method was used to evaluate the apoptosis of neuron; Western bolt method was used to detect the quantitative expression of bcl-2 and bax protein. RESULTS Compared with other groups, the BYHWD+ED group significantly reduced the neuronal apoptosis index (P<0.01), the quantitative expression of bcl-2 protein in BYHWD+ED group were more increased, while the quantitative expression of bax protein in BYHWD+ED group were more reduced(P<0.05). CONCLUSION The combination of BYHWD and ED can decrease the expression of bax, and increase bcl-2 expression to inhibit the nerve cell apoptosis and quicken the recovery of neural function, suggesting that the two agents play synergistic roles in protecting the brain from cerebral ischemic reperfusion injury.