黄连素对乳腺癌MCF-7细胞增殖和凋亡的影响 |
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引用本文: | 张亚军,钱立庭,陈昌杰.黄连素对乳腺癌MCF-7细胞增殖和凋亡的影响[J].安徽医科大学学报,2015,50(9):1276-1280. |
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作者姓名: | 张亚军 钱立庭 陈昌杰 |
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作者单位: | 安徽医科大学附属省立医院肿瘤放疗科,合肥 230001;蚌埠医学院第一附属医院肿瘤放疗科,蚌埠 233004;安徽医科大学附属省立医院肿瘤放疗科,合肥,230001;蚌埠医学院生化与分子生物学教研室,蚌埠,233030 |
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基金项目: | 安徽高校省级科学研究项目(KJ2013A184) |
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摘 要: | 目的 研究黄连素对人乳腺癌细胞株MCF-7的增殖和凋亡的影响以及机制. 方法 用不同浓度的黄连素 (0、10、20、40) g/ml处理人乳腺癌细胞株MCF-7,细胞培养72 h后,MTT法检测 MCF-7 细胞增殖抑制率;流式细胞术检测MCF-7细胞凋亡的影响;Western blot法检测JAK2、p-JAK2、p-STAT3、STAT3、 Bax、 Bcl-2、 Cleavage-PARP 和 Cleavage-Caspase3表达. 结果 黄连素呈浓度依赖性地诱导MCF-7细胞的增值抑制和凋亡,上调Bax、Cleavage-PARP和Cleav-age-Caspase3表达,下调 p-JAK2、p-STAT3 和 Bcl-2,对 JAK2和STAT3表达无明显影响. 但高表达p-STAT3的MCF-7细胞可以抑制黄连素的减少增殖和促进凋亡作用. 结论 黄连素诱导MCF-7细胞增殖抑制和凋亡的机制可能是通过调节JAK2/STAT3信号通路.
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关 键 词: | 黄连素 MCF-7 增殖 凋亡 JAK2/STAT3 |
Effect of berberine on proliferation and apoptosis of MCF-7 cells |
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Abstract: | Objective To investigate the effect and mechanisms of the Berberine on the human breast adenocarci-noma cell line MCF-7 . Methods MTT method was used to evaluate the proliferation effect of MCF-7 and the per-centage of apoptotic cells were determined by flow cytometric analysis. The expressions of JAK2, p-JAK2, p-STAT3, STAT3, Bax, Bcl-2, Cleavage-PARP and Cleavage-Caspase3 were detected by Western blotting. Results The results showed that BBR treatment decreased the activation of JAK2/STAT3 signal pathway and up-regulated the expression of Bax, Caspase3 and PARP activation with the decrease of the expression of Bcl-2. Wherefore, ex-pression of the constitutively active form of STAT3 could attenuate the effect of BBR on the MCF-7 cell. Conclusion Berberine can induces apoptosis and proliferation inhibition of breast adenocarcinoma cell lines of MCF-7 through inhibition of the JAK2/STAT3 signaling pathway. |
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Keywords: | berberine MCF-7 proliferation apoptosis JAK2/STAT3 |
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