| 酪酪肽对全肠内营养支持小鼠残留小肠代偿的影响 |
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| 引用本文： | 张伟,朱维铭,张坚,闫明文,李宁,黎介寿.酪酪肽对全肠内营养支持小鼠残留小肠代偿的影响[J].肠外与肠内营养,2008,15(1):11-15. |
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| 作者姓名： | 张伟 朱维铭 张坚 闫明文 李宁 黎介寿 |
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| 作者单位： | 南京大学医学院临床学院(南京军区南京总医院)解放军普通外科研究所,江苏南京,210002 |
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| 基金项目： | 江苏省医学重点人才基金 |
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| 摘 要： | 目的:通过建立广泛小肠切除(mSBR)的酪酪肽(PYY)基因敲除(Pyy-/-)小鼠模型,研究早期在TEN支持下PYY对残留小肠的代偿作用.方法:将30只Pyy-/-小鼠分为三组,每组10只.假手术(Sham)组仅横断小肠但不切除小肠;广泛小肠切除(mSBR)组切除50%小肠,保留近段空肠约1cm,远段回肠9ca;广泛小肠切除加PYY(mSBR-PYY)组切除50%小肠,皮内注射PYY1～36.所有小鼠术后2～7d均接受TEN.观察各组小鼠术后回肠黏膜的DNA和蛋白质含量、绒毛高度和隐窝深度、回肠隐窝细胞的增殖指数和绒毛上皮细胞的凋亡指数.结果:mSBR组小鼠同肠黏膜的DNA和蛋白质含量、绒毛高度、隐窝深度、回肠隐窝细胞的增殖指数和绒毛上皮细胞的凋亡指数,显著高于Sham组.mSBR-PYY组小鼠回肠黏膜的DNA和蛋白质含量、绒毛高度和隐窝深度,显著高于mSBR组;回肠隐窝细胞的增殖指数显著高于mSBR组;回肠绒毛上皮细胞的凋亡指数显著低于mSBR组.结论:在早期TEN支持下,PYY不仅能促进mSBR小鼠肠黏膜细胞的增殖,而且还能抑制其凋亡,在残留小肠的代偿中起重要作用.
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| 关 键 词： | 酪酪肽 广泛小肠切除 酪酪肽基因敲除小鼠 肠内营养 肠功能代偿 |
| 文章编号： | 1007-810X(2008)01-0011-05 |
| 修稿时间： | 2007年9月19日 |
The adaptive effects of residual intestine of peptide YY in mice with total enteral nutrition after massive small bowel resection |
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| ZHANG Wei,ZHU Wei-ming,ZHANG Jian,YAN Ming-Well,LI Ning,LI Jie-shou.The adaptive effects of residual intestine of peptide YY in mice with total enteral nutrition after massive small bowel resection[J].Parenteral & Enteral Nutrition,2008,15(1):11-15. |
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| Authors: | ZHANG Wei ZHU Wei-ming ZHANG Jian YAN Ming-Well LI Ning LI Jie-shou |
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| Institution: | ZHANG Wei,ZHU Wei-ming,ZHANG Jian,YAN Ming-wen,LI Ning,LI Jieshou(Research Institute of General Surgery,School of Medicine,Nanjing University/Nanjing General Hospital of Nanjing Military Comm,,PLA,Nanjing 210002,Jiangsu,China) |
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| Abstract: | Objective:The purpose of the present study was to explore the adaptive effects of residual intestine of peptide YY with early total enteral nutrition(TEN) in a massive small bowel resection(mSBR) model of PYY knockout(Pyy-/-) mice.Methods:Thirty,adult, male Pyy-/-mice were assigned into three experimental groups,each of 10:Sham mice(Sham group) underwent bowel transection and reanastomosis,mSBR mice(mSBR group) underwent a 50% small bowel resection and mSBR-PYY mice(mSBR-PYY group) underwent a 50% small bow... |
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| Keywords: | Peptide YY Massive small bowel resection PYY knockout mice Total enteral nutrition Intestinal adaptation |
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