逼尿肌细胞的原代培养和鉴定

目的建立逼尿肌细胞的原代培养及鉴定方法。方法应用Ⅱ型胶原酶消化分离逼尿肌细胞,在含20%胎牛血清DMEM培养液中培养,观察细胞形态和扩增情况,用a-SM-Actin进行免疫组化鉴定细胞类型。结果 逼尿肌细胞在培养18 h后开始贴壁在瓶底,4~5 d后可见细胞融合,8~10 d后可见细胞覆盖瓶底80%以上。a-SM-Actin免疫组化染色鉴定,光镜下观察梭形细胞胞浆内见纵行排列的棕黄色丝状物。结论该方法简单、易于掌握,短期内可获得大量高纯度的逼尿肌细胞。

Primary culture and identification of detrusor cells

Objective To establish a method of culturing and identifying detrusor cells.Methods Detrusor cells were digested with collagenase Ⅱ,and then were cultured in DMEM supplemented with 20% fetal bovine serum.Morphology and expansion of the cells were observed.Cells were identified by immunohistochemical methods with a-SM-Actin.Results After 18 hours,the detrusor cells adhered at the bottom of the culture bottle.After 4-5 days,the detrusor cells were observed to fuse.After 8-10 days,the detrusor cells covered over 80% of the bottom of the culture bottle.Immunohistochemical identification showed the microtubule brown-yellow mesh in cytoplasm was observed by light microscope.Conclusion The cultural method for detrusor cells is simple and convenient to be learnt and to get fruitful cells in short time.