Natural killer cells do not mediate facial motoneuron survival after facial nerve transection |
| |
Authors: | Byram Susanna C Serpe Craig J Pruett Stephen B Sanders Virginia M Jones Kathryn J |
| |
Affiliation: | Department of Cell Biology, Neurobiology and Anatomy, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL 60153, USA. scoers@lumc.edu |
| |
Abstract: | The goal of the current study was to determine if natural killer (NK) cells mediate facial motoneuron (FMN) survival following injury. Wild-type (WT), perforin/recombinase activating gene-2 knockout (pfp/RAG-2 KO), and common gamma-chain (gammac)/RAG-2 KO mice received a right facial nerve axotomy. In WT mice, FMN survival was 86+/-1.0% relative to the contralateral control side. In contrast, pfp/RAG-2 and gammac/RAG-2 KO mice exhibited significant decreases in FMN survival ( approximately 20% and approximately 30%, respectively), relative to WT. Reconstitution of pfp/RAG-2 and gammac/RAG-2 KO mice with normal NK cells alone, failed to restore FMN survival levels to those of WT, but did restore functional lytic activity against YAC-1 cells. Reconstitution of pfp/RAG-2 and gammac/RAG-2 KO mice with splenocytes, and pfp/RAG-2 KO mice with CD4+ T-lymphocytes alone or in combination with NK cells, restored FMN survival levels to those of WT. Thus, NK cells appear to not be a component of immune cell-mediated rescue of motoneurons from axotomy induced cell death. |
| |
Keywords: | Natural killer cells NK cells Neuro-immune interactions Neuroimmunology Facial nerve FMN Innate immunity Reconstitution Neuronal survival Peripheral nerve injury |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|