酒精性脂肪肝大鼠肠源性内毒素血症模型的建立 |
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引用本文: | 张军会,王永辉,周然,王悦尧. 酒精性脂肪肝大鼠肠源性内毒素血症模型的建立[J]. 实用肝脏病杂志, 2014, 0(1): 15-17 |
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作者姓名: | 张军会 王永辉 周然 王悦尧 |
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作者单位: | [1]成都中医药大学,成都市610075 [2]山西中医学院,成都市610075 |
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摘 要: | 目的:建立酒精性脂肪肝大鼠肠源性内毒素血症模型。方法采用梯度酒精灌胃法早晚两次灌胃,并以10%酒精为饮料,建立大鼠酒精性脂肪肝模型。分别于3w和6w检测肝脂肪变、肝内炎症、肝功能和血清内毒素水平。结果模型组自6w出现显著的肝脂肪变,肝指数(3.6±0.2)、肝脂肪变积分(3.0±0.9)和炎症积分(1.0±0.6)均显著高于同期对照组水平[分别为(3.1±0.1)、(0.0±0.0)和(0.0±0.0),P<0.05];在6周时模型动物血浆内毒素、血清D-乳酸、二胺氧化酶和AST分别为(1435.6±52.9)pg/ml、(20.7±5.4)mmol/L、(25.5±2.0)U/L和(124.5±13.2) U/L,较正常组均明显升高[分别为(89.9±10.5)pg/ml、(5.0±1.1)mmol/L、(7.4±1.7)U/L和(40.4±15.2)U/L,P<0.05]。结论用该方法连续6w成功建立单纯性酒精性脂肪肝肠源性内毒素血症模型。
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关 键 词: | 酒精性脂肪肝 肠源性内毒素血症 肠黏膜通透性 大鼠 |
Establishment of experimental model of alcoholic fatty liver accompanied with intestinal endotoxemia in rats |
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Affiliation: | Zhang Junhui,Wang Yonghui,Zhou Ran,et al.( Chengdu University of Traditional Chinese Medicine,Chengdu 610075 ,Sichuan Province,China) |
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Abstract: | Objective To establish an experimental model of alcoholic fatty liver accompanied with intesti-nal endotoxemia in rats. Methods A rat model of alcoholic fatty liver was established by gradient ethanol gavage method;The liver steatosis,intrahepatic inflammation,liver function tests and serum lipopolysaccharide were deter-mined at 3 and 6 weeks of experiment. Result Significant liver steatosis was observed after 6 weeks of ethanol administration in the model rats;The liver index,liver steatosis scores,inflammation scores in model rats were(3.6± 0.2),(3.0±0.9)and (1.0±0.6),respectively, significantly higher than those in normal controls [(3.1±0.1),(0.0±0.0) and (0.0 ±0.0),respectively,P<0.05)];Similarly,serum lipopolysaccharide,D-lactate,diamine oxidase and aspertate aminotransferase levels in model rats at 6 months were (1435.6 ±52.9) pg/ml,(20.7 ±5.4) mmol/L,(25.5 ±2.0)U/L and (124.5±13.2) U/L,which were also significantly higher than those in normal controls[(89.9±10.5) pg/ml,(5.0± 1.1)mmol/L,(7.4±1.7) U/L and(40.4±15.2) U/L,respectively,P<0.05]. Conclusion A rat model of alcoholic fatty liver accompanied with intestinal endotoxemia can be successfully establish by using gradient ethanol gavage for 6 weeks. |
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Keywords: | Alcoholic fatty liver Intestinal endotoxemia Permeability of intestinal mucosa Rats |
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