肝癌患者血清microRNA-21水平变化 |
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引用本文: | 郝清亚,王以浪,姚登福,缪亚军,印滇. 肝癌患者血清microRNA-21水平变化[J]. 实用肝脏病杂志, 2014, 0(1): 68-70 |
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作者姓名: | 郝清亚 王以浪 姚登福 缪亚军 印滇 |
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作者单位: | [1]江苏省南通市第一人民医院肝胆外科,226001 [2]江苏省南通市第一人民医院肿瘤科,226001 [3]南通大学附属医院临床医学研究中心,226001 |
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基金项目: | 国家国际科技合作专项(2013DFA32150);南通市科技计划项目(SI1904/K2010026) |
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摘 要: | 目的:分析不同肝病患者血清microRNA-21(miR-21)水平,以探讨miR-21对肝细胞癌(HCC)的诊断价值。方法以实时定量逆转录PCR法检测正常人、慢性乙型肝炎(CHB)、肝硬化和HCC患者(各组均为25例)血清miR-21水平,分析miR-21水平与HCC临床病理学特征的关系。结果正常人、CHB和肝硬化患者血清miR-21相对水平分别为(1.1±1.7)、(2.3±2.6)和(2.8±2.5),而HCC患者为(22.6±4.4),显著高于前三组(P〈0.001);HCC患者术后1w和1m血清miR-21相对水平分别为(18.4±3.5)和(3.1±2.7),均较术前显著降低(P<0.001);HCC患者血清miR-21水平与肿瘤大小、癌栓以及HBV感染相关,与肿瘤分化程度、数目和血清AFP水平无相关性。结论 miR-21在HCC患者血清中显著升高,可能作为HCC早期诊断的潜在标志。
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关 键 词: | 肝细胞癌 miRNA-21 血清 诊断 MicroRNA-21 |
Serum level of microRNA-21 in patients with hepatocellular carcinoma |
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Affiliation: | Hao Qingya, Wang Yilang, Yao Denffu,et al. (First People's Hospital,Natong 226001,Jiangsu Province,China) |
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Abstract: | Objective To evaluate serum level of microRNA-21 (miR-21) in normal persons and patients with different liver diseases,and to investigate its effect in the diagnosis of hepatocellular carcinoma(HCC). Methods The serum level of miR-21 in healthy controls (n=25),patients with chronic hepatitis B(CHB,n=25), cirrhosis (n=25),and HCC (n=25) were measured by real-time quantitative RT-PCR. The relationships between serum miR-21 levels and clinical pathological features of HCC were also investigated. Results The relative serum levels of miR-21 in normal controls,CHB and cirrhotic patients were (1.1±1.7),(2.3±2.6) and (2.8±2.5),respec-tively,all of which were significantly lower than that in HCC patients(22.6±4.4),P〈0.001;The relative serum levels of miR-21 in HCC patients one week and one month after surgery were(18.4±3.5) and (3.1±2.7),respectively,sig-nificantly lower than that before operation (22.6±4.4),P〈0.001;Serum levels of miR-21 were correlated with tumor size,tumor thrombosis and HBV infection,but not with tumor numbers,tumor differentiation and serum AFP levels. Conclusions Serum levels of miR-21 is elevated in HCC patients and might be used as a potential biomarker for the early diagnosis of HCC. |
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Keywords: | Hepatocellular carcinoma Serum Diagnosis |
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