Effect of dibromotetrafluoroethane inhalation on hepatic drug metabolism in mice

Inhalation of dibromotetrafluoroethane, 0.63 to 1.0%, for 5 hr daily, for 3 or 4 days, reduced hexobarbital sleeping time and zoxazolamine paralysis time 2-fold in mice. Increased metabolism of hexobarbital and zoxazolamine by the hepatic 9000 g microsomal supernatant fraction prepared from exposed mice correlated well with the effects determined in vivo. Addition of 2,4-dichloro-6-phenyl-phenoxyethyldiethylamine (SKF 525-A) to the hepatic 9000 g microsomal supernatant fraction prevented the dibromotetrafluoroethane-induced increase in hepatic drug metabolism.