Effect of microneedle treatment on the skin permeation of a nanoencapsulated dye |
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| Authors: | Yasmine A. Gomaa Labiba K. El‐Khordagui Martin J. Garland Ryan F. Donnelly Fiona McInnes Victor M. Meidan |
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| Affiliation: | 1. Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt;2. School of Pharmacy, Queen's University Belfast, Medical Biology Centre, Belfast;3. Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), University of Strathclyde, Glasgow, UK |
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| Abstract: | Objectives The aim of the study was to investigate the effect of microneedle (MN) pretreatment on the transdermal delivery of a model drug (Rhodamine B, Rh B) encapsulated in polylactic‐co‐glycolic acid (PLGA) nanoparticles (NPs) focusing on the MN characteristics and application variables. Methods Gantrez MNs were fabricated using laser‐engineered silicone micro‐mould templates. PLGA NPs were prepared using a modified emulsion–diffusion–evaporation method and characterised in vitro. Permeation of encapsulated Rh B through MN‐treated full thickness porcine skin was performed using Franz diffusion cells with appropriate controls. Key findings In‐vitro skin permeation of the nanoencapsulated Rh B (6.19 ± 0.77 µg/cm2/h) was significantly higher (P < 0.05) compared with the free solution (1.66 ± 0.53 µg/cm2/h). Mechanistic insights were supportive of preferential and rapid deposition of NPs in the MN‐created microconduits, resulting in accelerated dye permeation. Variables such as MN array configuration and application mode were shown to affect transdermal delivery of the nanoencapsulated dye. Conclusions This dual MN/NP‐mediated approach offers potential for both the dermal and transdermal delivery of therapeutic agents with poor passive diffusion characteristics. |
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| Keywords: | microneedles nanoparticles Rhodamine B |
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