艾炷灸贴预处理对佐剂性关节炎大鼠早期和继发期血清白细胞介素-6、睾酮、生长激素及皮质醇的影响

目的:探讨艾炷灸贴预处理对佐剂性关节炎(AA)大鼠早期与继发期血清炎性因子和激素的影响及保护机制。方法:将40只Wistar大鼠随机分为正常组、模型早期组和继发组、预灸贴早期组和继发组。于造模前用中药膜剂贴敷大鼠“大椎”穴后加艾炷灸,采用弗氏完全佐剂制备大鼠模型,用放射免疫法测定各组大鼠血清白细胞介素-6(IL-6)、睾酮(T)、生长激素(GH)及皮质醇(CORT)的含量。结果:血清IL-6和GH含量造模后均明显升高(P<0.01);与同期模型组相比,预灸贴继发组均明显下降(P<0.05)。血清T含量造模后早期组明显降低(P<0.05),继发组变化不大;预灸贴各组较同期模型组均显著升高(P<0.01,0.05)。血清CORT含量造模后显著升高(均P<0.01),而预灸贴继发组与正常组比无明显差异(P>0.05)。结论:艾炷灸贴预处理可减轻随后佐剂性关节炎大鼠原发足局部病理损伤,降低血清炎性因子IL-6的水平,对血清T、GH及CORT等内分泌激素的紊乱状态具有良性调节作用。

Influence of Acupoint-application and Moxibustion Pretreatment on Serum IL-6, T, GH and CORT Levels in Rats with Adjuvant Arthritis at the Early and Secondary Stages

Objective: To observe the effects of acupoint-application and moxibustion pretreatment on serum inflammatory cytokine and hormones of rats with adjuvant arthritis at early and secondary stages.Methods: Forty Wistar rats were randomly divided into normal,early-stage model,secondary-stage model groups,early-stage pretreatment and secondary-stage pretreatment groups.Arthritis model was established by injecting Freund's complete adjuvant(FCA,(0.1 mL)) into the rat's right hind-paw.After removing the hair and before injecting FCA respectively,a plaster made of Herba Epimedii,etc. and an ignited moxa cone(placed on the plaster) were applied to "Dazhui"(GV 14),once every other day,8 times altogether.Rats of the 4 early-stage and secondary-stage groups were killed on the 3~(rd) day and 16~(th) day after injection of FCA respectively,and those of normal group also killed for collecting blood samples.The contents of serum IL-6,testosterone(T),growth hormone(GH) and cortisol(CORT) were assayed respectively with radioimmunoassay according to the instructions of reagent kits.Results: Compared with normal group,the contents of serum IL-6,GH and CORT of early-stage-model and secondary-stage-model groups increased significantly((P<0.01)),and those of early-stage pretreatment groups,and serum T and GH of secondary-stage pretreatment groupsalso increased remarkably((P<0.05,0.01));but T level of early-stage model group decreased significantly((P<0.05));while in comparison with the corre-sponding model groups,the contents of T in early-stage and secondary-stage pretreatment groups increased considerably((P<0.05,0.01));and those of serum IL6 and GH lowered markedly((P<0.05));no significant differences were found among the 2 model and 2 pretreatment groups((P>0.05)).Conclusion: Preconditioning of acupoint-application and moxibustion at GV 14 can effectively suppress arthritis induced increase of serum IL-6 and GH levels and decrease of T to modulate inflammatory cytokine and endocrine hormones, playing a protective role in adjuvant arthritis rats.