转人白细胞介素-12基因膀胱癌瘤苗的抗肿瘤作用

目的 制备人白细胞介素—12(hIL-l2)转基因膀胱癌瘤苗，观察其特异性抗肿瘤免疫作用。方法 经逆转录病毒载体将hIL—12编码基因导入膀胱癌细胞株MBT—2中，用聚合酶链反应(PCR)技术检测目的基因是否整合入MBT—2基因组中，用四甲基偶氮唑盐(MTT)法检测hIL—12活性，将转基因细胞接种到36只荷瘤小鼠，检测细胞毒T细胞杀伤活性。结果 hIL—12 cDNA经逆转录病毒载体导入MTT—2细胞中后，hIL—12基因已完整、稳定地整合到MBT—2基因组中，使MBT—2细胞具有分泌hIL—12活性，每24h达32．8mol/L以上。转基因细胞接种荷瘤小鼠后，可见瘤体变小(P＜0．01)，细胞毒T细胞杀伤活性增强。结论 转hIL—12基因瘤苗，能够诱导小鼠的特异性抗肿瘤免疫反应。

Anti-tumor effect of bladder cancer vaccine transferred with hIL-12 gene

Objective To prepare the bladder cancer vaccine transferred with hIL12 gene to test its anti-tumor response.Methods The hIL12 gene was transferred into the bladder cancer cell by the retrovirus vector in 36 mice.The gene integration was confirmed by PCR.The hIL12 activity was detected by MTT technique.The anti-tumor response was revealed through the CTL activity.Results The hIL-12 gene was successfully integrated into the target cell genome.The vaccine cell could secrete hIL-12 (>32.8 mol/L every 24 h).The tumor weight was decreased and the CTL activity was increased significantly by the tumor vaccine ( P <0.01).Conclusion Cancer vaccine integrated with hIL12 can induce specific anti-tumor response in target cell.