| 地鳖虫纤溶成分的分离纯化和活性测定 |
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| 引用本文： | 韩雅莉,李张伟.地鳖虫纤溶成分的分离纯化和活性测定[J].中药材,2006,29(8):765-767. |
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| 作者姓名： | 韩雅莉 李张伟 |
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| 作者单位： | 1. 广东工业大学轻工化工学院,广东,广州,510090
2. 汕头大学理学院,广东,汕头,515063 |
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| 基金项目： | 广东省自然科学基金;广东工业大学校科研和教改项目 |
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| 摘 要： | 本文通过硫酸铵分级分离、DEAE-纤维素柱和非变性PAGE制备电泳等方法从雌性地鳖虫EupolyphagesinensisW alker活体内分离纯化得到三种纤溶活性成分:EFF-1、EFF-2和EFF-3,分子量分别为41 kd、32.9 kd和30.6 kd,比活力分别为171.3 U/mgpr、234.0 U/mgpr和148.5 U/mgpr,其中EFF-2和EFF-3兼有纤溶酶激活物和纤溶酶活性。
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| 关 键 词： | 地鳖虫 纤溶酶激活物 纤溶酶 制备电泳 |
| 文章编号： | 1001-4454(2006)08-0765-03 |
| 收稿时间： | 2005-12-20 |
| 修稿时间： | 2005年12月20 |
Purification and Activity Calculated of Fibrinolyric Factors from Eupolyphaga sinensis |
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| HAN Ya-li,LI Zhang-wei.Purification and Activity Calculated of Fibrinolyric Factors from Eupolyphaga sinensis[J].Jorunal of Chinese Medicinal Materials,2006,29(8):765-767. |
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| Authors: | HAN Ya-li LI Zhang-wei |
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| Institution: | 1. Bioengineering Department, Guangdong University of Technology, Guangzhou 510090, China; 2. College of Science, Shantou U- niversity, Shantou 515063, China |
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| Abstract: | Three kinds ( EFF-1, EFF-2 and EFF-3) of fibrinolytic factor were separated by ammonium sulphate precipitation, DEAE-cellulose and preparative PAGE electrophoresis from female Eupolyphaga sinensis Walker. Their molecular weights were proved to be 41kd, 32.9 kd and 30.6 kd respectively with SDS-PAGE electophoresis. Their Activities as plasminogen activator were 171.3 U/mg, 234.0 U/mg and 148.5 U/mg. In addition, EFF-2 and EFF-3 were not only fibrinolytic activities but also have plasminogen activator on fibrinous plate lacked of plasminogen . There had been no such components of plasminogen activator and fiberinolytic enzyme from Eupolyphaga sinensis reported yet. |
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| Keywords: | Eupolyphaga sinensis Walker Plasminogen activator Fibrinolysin Preparative PAGE electrophoresis |
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