GATA-2过表达对小鼠胎肝造血干细胞功能的影响

本研究探讨GATA-2过表达对小鼠胎肝造血干细胞功能的影响。应用带GFP绿荧光的逆转录病毒载体介导的病毒感染实验将GATA-2基因引入小鼠胎肝细胞,通过流式免疫荧光术、细胞周期检测及集落形成实验等技术手段研究这些GATA-2过表达胎肝造血干细胞的生物学功能变化。研究结果表明:过表达GATA-2的胎肝细胞中Lin-Sca1+C-Kit+(LSK)细胞比例显著增加,LSK细胞周期未受太大影响,但其定向克隆形成能力受抑。结论:过表达GATA-2引起小鼠胎肝造血干细胞LSK比例增加,并抑制其定向分化能力,其机制可能与HES-1表达上调导致细胞在干/祖细胞阶段受阻滞有关。

Effect of GATA-2 Overexpression on Function of Mouse Fetal Liver Hematopoietic Stem Cells

This study was aimed to investigate the effect of GATA-2 over-expression on function of mouse fetal liver hematopoietic stem cells.GATA-2 was introduced into mouse fetal liver cells via retrovirus mediated transduction with GFP as a detecting marker.Flow cytometry,colony-forming assay and cell cycle assay were used to detect the biologic changes of these retrovirus infected mouse fetal liver hematopoietic stem cells.The results showed that GATA-2 over-expression increased the Lin^-Sca1^＋C-Kit^＋（LSK） population dramaticly.Cell cycle of LSK cells didn′t show abnormal,while colony forming ability decreased significantly.These data indicated that GATA-2 over-expression inhibited definitive differentiation of mouse fetal liver hematopoietic stem cells.It is concluded that over-expression of GATA-2 can significantly raise the LSK cell proportion in mouse fetal liver and inhibit the differentiation capability,the underlying mechanisms may be related to up-regulation of Hes-1,which may lead to the blocking of cell differentiation at the stem/progenitor cell stage.