全血手工制备浓缩血小板后的血浆再制备冷沉淀的质量评价

目的评价室温新鲜全血白膜法制备浓缩血小板后的血浆再制备冷沉淀的质量。方法实验组为24例,新鲜全血(400 mL)置室温于<8 h用白膜法制备浓缩血小板后所得的血浆,冰冻保存。对照组1为12例,常规制备新鲜冰冻血浆,冰冻保存。对照组2为12例,新鲜冰冻单采血浆,血浆单采完毕分装为200 mL/袋并立即冰冻保存。3组血浆按常规制备冷沉淀,评价其质量:外观、凝血因子FⅧ及Fib的含量;血细胞残留量。结果 3组冷沉淀外观均正常;WBC含量在3组间无统计学意义。与对照组1比较:实验组凝血因子FⅧ(81.76±34.07)IU较低,Fib(202.63±48.58)mg及Plt(7.81±5.81)×109均较高。与对照组2比较:实验组凝血因子FⅧ含量相当,Fib(202.63±48.58)mg较高、Plt(7.81±5.81)×109较低。结论全血来源的制备浓缩血小板后的冰冻血浆还可以用于冷沉淀的制备,其质量符合国家标准。

Quality of cryoprecipitate prepared from the plasma after separation of platelets by buffy coat method

Objective To investigate the quality of cryoprecipitate prepared from plasma after preparation of buffy coat derived platelet concentrats.Methods The experimental group consisted of 24 cryoprecipitates prepared from plasma after platelet concentrates were prepared by buffy coat method,using whole blood stored at room temperature for less than 8 h.Control group 1 consisted of cryoprecipitates prepared from regular fresh frozen plasma,prepared from whole blood stored at 4 ℃ within 8 h of whole blood collection.Control group 2 consisted of cryoprecipitates prepared from apheresis plasma frozen within 2 h of collection.All the plasma units were frozen for 3 d before preparation of cryoprecipitate.The quality of cryoprecipitate was evaluated by appearance,FⅧ and fibrinogen(Fib) content,and residual blood cells.Results The cryoprecipitates of all 3 groups showed normal appearance.There was no difference in residual WBC counts between the three groups.Compared to control group 1,the clotting factor FⅧ in the experimental group was lower,but the Fib and platelet counts were higher.There was no difference in FⅧ between the experimental group and control group 2.However,Fib level was obviously higher and platelet count was significantly lower in the former group.Conclusion The quality of cryoprecipitate prepared from plasma after preparation of platelet concentrats from whole blood stored at room temperature within 6 h of whole blood collection could meet the national criteria.