白膜放置时间对制备手工浓缩血小板质量的影响

目的评价全血当天分离的白膜(BC)在不同时间分离制备的浓缩血小板(PC)的质量,为手工制备PC提供参考。方法将80袋400 mL全血于采集6 h内分离出BC,将5袋同血型BC由无菌接驳机对接合并成1袋(1个治疗量)后,再均分在3个血小板保存袋内,1袋即刻(0 h组)轻离心分离制备PC,另2袋在22℃血小板保存箱分别振摇4 h(4 h组)和16 h(16 h组)后再分离PC。对所有标本留样进行血小板质量检测,包括Plt、血小板回收率、CD62P、聚集率、RBC混入量、WBC混入量、FHb含量。结果 3组PC制剂RBC混入量、Plt、血小板回收率、CD62P、聚集率差异无统计学意义;WBC混入量:0 h(6.76±1.29)和4 h组变化不明显,16 h(3.78±0.45)组降低明显(P<0.05);FHb含量:随BC处理时间延长有增高趋势,16 h(65.62±11.11)与0 h(33.45±6.95)比差异具统计学意义(P<0.05)。结论随BC放置时间延长对制备PC制剂的质量有一定影响。

Effect of holding buffy coats before platelet preparation on the quality of platelet concentrates

Objective To evaluate the quality of platelet concentrates(PCs) prepared from buffy coats held for different time periods.Methods Buffy coats(BCs)(n=80) were prepared from 400 mL whole blood(WB) units within 6 h of collection.Every 5 BCs were pooled and then each pool were divided into three equal bags.One bag was taken to prepare PCs immediately(0 h group),and the other two bags of BCs were agitated in platelet incubator,and agitated for 4 h(4 h group),and 16 h(16 h group) at 22℃ before PC preparation.The in vitro quality of platelets was investigated.Results Platelet,RBC counts showed little difference between these groups.In comparison to 0 h and 4 h groups,the decrease of WBC counts in the 16 h group was significant.Free hemoglobin increased as the agitation time extended.Conclusion The agitation time of BCs may affect the quality of platelet concentrates.