维甲酸受体-β等肿瘤分子标志物在食管正常及异常组织中的表达及意义

目的 研究食管癌高发区人群的正常食管上皮、癌前病变及癌组织活检标本中,维甲酸受体-β(RAR-β)mRNA、p16、p53和Ki67蛋白的异常表达及其与食管癌发生的关系。方法 全组397例标本,其中食管正常黏膜组织25例,轻度不典型增生69例,中度不典型增生106例,重度不典型增生51例,原位癌78例,鳞状细胞癌68例。应用RNA原位杂交技术检测RAP-β mRNA的水平,应用免疫组织化学技术检测p16、p53和Ki67蛋白的表达情况。结果 在正常黏膜、轻度、中度、重度不典型增生、原位癌及鳞状细胞癌组织中,RAR-β mRNA表达检出率分别为96．0%、89．9%、67．9％、68．6％、62．8％和63．2％;p16表达检出率分别为88．0％、71．0％、64．2％、51．0%、53．8％和52．9%;p53异常表达检出率分别为4．0％、39．1％、57．5%、52．9％、67．9％和69．1％;Ki67异常表达检出率为0、40．6％、61．3％、58．8%、59．0％和75．0%。结论 4种肿瘤生物学标志物在食管上皮中、重度不典型增生和原位癌组织中的表达,未出现明显差异,而只表现为形态学上的差异。

Expression of retinoic acid receptor-beta mRNA and p16, p53, Ki67 proteins in esophageal carcinoma and its precursor lesions

OBJECTIVE: To study the expression of retinoic acid receptor-beta (RAR-beta) mRNA and p16, p53, Ki67 proteins in squamous-cell carcinoma of the esophagus and its precursor lesions in a high risk population. METHODS: A total of 397 tissue specimens were collected from individuals with normal mucosa (NM, n = 25), mild dysplasia (MiD, n = 69), moderate dysplasia (MoD, n = 106), severe dysplasia (SD, n = 51), carcinoma in situ (CIS, n = 78), and squamous-cell carcinoma (SC, n = 68). Expression of RAR-beta mRNA was detected by in situ hybridization, and that of p16, p53 and Ki67 proteins by immunohistochemistry. RESULTS: The frequencies of RAR-beta mRNA expression in NM, MiD, MoD, SD, CIS and SC were 96.0%, 89.9%, 67.9%, 68.6%, 62.8%, and 63.2%, respectively. The frequencies of p16 expression were 88.0%, 71.0%, 64.2%, 51.0%, 53.8% and 52.9%; those of p53 expression were 4.0%, 39.1%, 57.5%, 52.9%, 67.9% and 69.1%; those of Ki67 expression were 0, 40.6%, 61.3%, 58.8%, 59.0% and 75.0%, respectively. CONCLUSION: There are no significant differences in four biomarkers expression between carcinoma of the esophagus and its precursor lesions.