| 载体介导的RNAi抑制乙肝病毒S基因的研究 |
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| 引用本文： | 周豪杰,汪传喜,滕青,聂咏梅. 载体介导的RNAi抑制乙肝病毒S基因的研究[J]. 海南医学, 2010, 21(20): 4-6 |
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| 作者姓名： | 周豪杰 汪传喜 滕青 聂咏梅 |
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| 作者单位： | 广州血液中心质量控制科,广东,广州,510095;广州血液中心质量控制科,广东,广州,510095;广州血液中心质量控制科,广东,广州,510095;广州血液中心质量控制科,广东,广州,510095 |
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| 基金项目： | 广州市医药卫生科技一般引导项目,广东省自然科学基金项目 |
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| 摘 要： | 目的构建针对乙肝病毒S基因的RNAi表达载体,观察其对HBV表达的影响。方法合成针对HBVS基因的RNAi寡核苷酸,克隆入pSUPER载体,与pHBV质粒共转染HepG2细胞。ELISA和Westernblot检测HBsAg表达。结果成功构建针对HBVS基因的RNAi表达载体pSUPER-S1和pSUPER-S2对HBsAg的抑制率分别为79.0%和43.2%,无关序列的RNAi表达载体无此作用。结论载体介导的RNAi能高效、特异地抑制HBVS基因的表达。
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| 关 键 词： | 乙型肝炎病毒 RNA干扰 乙肝病毒表面抗原 |
Inhibitory efects of vector-based RNAi on S gene expression of hepatitis B virus |
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| Affiliation: | ZHOU Hao-jie,WANG Chuan-xi,TENG Qing,et al.Department of Quality Control,Guangzhou Blood Center,Guangzhou 510095,Guangdong,CHINA |
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| Abstract: | Objective To construct RNAi expressing vectors targeting HBV S gene and to evaluate the inhibitory effects on HBV replication.Methods Two sequences targeting HBV S gene were cloned into pSUPER vector and then were cotransfected with pHBV into HepG2 cells.HBsAg expression was assayed by ELISA and Western blot.Results PSUPER-S1 and pSUPER-S2 RNAi expressing vectors were successfully constructed and inhibited HBsAg in HepG2 cells by 79.0% and 43.2%,Whereas irrelevant RNAi expressing vector did not show inhibitory effects on the HBsAg expression.Conclusion Vector-based RNAi can inhibit HBV replication effectively and specifically. |
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| Keywords: | Hepatitis B virus RNA interference HBsAg |
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