针刺对痛经小鼠止痛作用及其机制的研究

目的：探讨针刺治疗痛经的作用机制。方法：成年未孕小鼠随机分为正常组（N）、模型组（M）、针刺组（A）及药物组（P）。M组、A组和P组均采用乙烯雌酚和催产素造模。A组于造模第7d针刺“三阴交”“地机”,每日1次,连续治疗5d;P组于造模第7d予益母草膏0．6mg／g灌胃,连续5d。观察各组小鼠扭体潜伏期及30min内扭体数;逆转录聚合酶链反应（RT—PCR）检测各组小鼠子宫组织中催产素受体（OctR）及血管加压素受体（VasR）mRNA表达的差异。结果：A组及P组扭体潜伏期较M组延长（P〈0．05）;A组及P组30min内扭体数低于M组（P〈0．05）;M组OctR、VasR mRNA表达水平与另3组的差异亦有统计学意义（P〈0．05,P〈0．01）。结论：针刺在一定程度上可改善痛经症状,其作用机制可能与针刺对小鼠体内激素介导的受体表达的调控有关。

Study on effects of acupuncture on mice dysmenorrhea model and the mechanism

OBJECTIVE: To probe the mechanism of acupuncture in treatment of dysmenorrhea. METHODS: Adult mice with no pregnancy were randomly divided into a normal group, a model group, an acupuncture group and a medication group. The model group, the acupuncture group and the medication group were modeled by Diethylstilbestrol and Ocytocin. For the acupuncture group, at the 7th day of modeling, acupuncture was given at "Sanyinjiao" (SP 6), "Diji" (SP 8), once a day, for 5 days; and at the 7th day of modeling, Yimucao Gao 0.6 mg/g was given intragastrically to the medication group for 5 days. The stretching latent period and the number of stretching within 30 min were observed, and mRNA levels of ocytocin receptor (OctR) and vasopressin receptor (VasR) in the uterus tissue were detected with RT-PCR method. RESULTS: Compared with the model group, the stretching latent period extended (P < 0.05) and the number of stretching within 30 min significantly decreased (P < 0.05); and there were significant differences in the mRNA levels of ocytocin receptor and vasopressin receptor in the uterus tissue in the model group as compared with those in other 3 groups (P < 0.05, P < 0.01). CONCLUSION: Acupuncture can improve the dysmenorrheal symptom to a certain extent, and the mechanism is possibly related to regulative effects of acupuncture on hormones-mediating receptors in mice.