溶血磷脂酰胆碱对ECV304细胞中血管内皮生长因子表达的影响(英文)

目的:研究溶血磷脂酰胆碱(LPC)对内皮细胞(EC)中血管内皮生长因子(VEGF)表达的影响。方法:在人脐静脉内皮细胞株ECV04培养基中加入不同浓度的LPC,培养不同时间,用基础酶联免疫吸附实验(ELISA)检测各组EC条件培养基中VEGF蛋白含量;用免疫组织化学法检测EC中VEGF蛋白及其受体的表达;用原位杂交检测VEGF信使核糖核酸(VEGF mRNA)的表达。结果:培养的ECV304能表达VEGF受体,在胞浆内呈棕色颗粒,当LPC刺激后,阳性增强。原位杂交结果显示,培养的ECV304中未见VEGF mRNA的表达,当LPC刺激后可见VEGF mRNA的高表达,在胞浆内呈棕色颗粒。ELISA结果显示LPC可使ECV304条件培养基中VEGF蛋白含量明显增加,且具有时间和剂量依赖性。结论:LPC能诱导ECV304表达高水平的VEGF。 (责任编辑 吕静)

Effect of lysophosphatidylcholine on expression of vascular endothelial growth factor in ECV304 cells

AIM: To determine the effect of lysophosphatidylcholine (LPC) on the expression of vascular endothelial growth factor (VEGF) in human umbilical veins endothelial cell line (ECV304). METHODS: VEGF protein production from ECV304 cells was determined by enzyme- linked immunosorbent assay (ELBA). VEGF protein and its receptor were also examined by immunohistochemical staining. After culturing for 24 h with LPC, VEGF mR-NA expression in ECV304 was examined by in situ hybridization. RESULTS: Cultured ECV304 cells could express VEGF receptor and a strong expression of VEGF receptor was induced by LPC 5 mg/L. LPC upregula ted VEGF protein secreted from the ECV304 cells in a tirne-and concentration-dependent manner and LPC also stimulated VEGF mRNA expression in the ECV304 cells. CONCLUSION: LPC could induce a strong expression of VEGF in ECV304 cells.