高浓度葡萄糖抑制兔胸主动脉内皮依赖的血管舒张(英文)

目的:研究高浓度葡萄糖是否能抑制兔胸主动脉内皮依赖的血管舒张以及可能的机制。方法:利用器官组织浴动脉环法测定血管张力,观察去除内皮、NO合酶抑制剂L-NMMA,不同浓度的葡萄糖对乙酰胆碱(ACh)产生的内皮依赖的和硝普钠(SNP)产生的非内皮依赖的血管舒张功能的影响以及维生素C和膜渗透性抗氧化剂MnTMPyP对高浓度葡萄糖作用的影响。结果:ACh和SNP均产生浓度依赖的血管舒张效应,高浓度葡萄糖明显抑制ACh产生的血管舒张,而对SNP的作用无明显影响,维生素C和MnTMPyP不能逆转高浓度葡萄糖对ACh产生的血管舒张的抑制作用。结论:高浓度葡萄糖抑制内皮依赖的血管舒张,这种作用不是通过增加氧自由基的产生介导的。

High concentration of glucose inhibits endothelium-dependent vasorelaxation of rabbit aortic artery

AIM: To determine whether high concentration of glucose inhibits endothelium-dependent vasorelaxation of rabbit aortic artery and possible mechanisms. METHODS : The organ-bath of rings of rabbit aorta was used to determine changes of tension of vessel in response to different concentrations of acetylcholine (ACh) and sodium nitroprusside (SNP) after removal of endo-thelium, coincubated with nitric oxide synthase (NOS) inhibitor L-NMMA, different concentrations of glucose, vitamin C, and cell-permeable superoxide dismutase-mimetic and oxygen-derived free radical scavenger manganese (III) tetrakis (1-methyl-4-pyridyl) porphyrin (MnTMPyP). RESULTS: ACh induced concentration-and endothelium-dependent aortic vasorelaxation. High concentration of glucose markedly inhibited this vasorelaxation, and vitamin C and MnTMPyP could not antaganize this inhibitory effect by nigh concentrations of glucose. SNP induced concentration-dependent and endothelium-independent vasorelaxation, and high concentration of glucose had no effect on the vasorelaxation by SNP. CONCLUSION: High concentration of glucose inhibited endothelium-dependent vasorelaxation and this effect was unlikely mediated through activating oxygen-derived free radical production.