芝麻素对动脉粥样硬化斑块及主动脉壁VCAM-1表达的影响

目的观察比较芝麻素和阿托伐他汀对兔实验性动脉粥样硬化（AS）斑块形成及主动脉壁细胞黏附分子1（VCAM-1）表达的影响,探讨芝麻素在预防和治疗AS中的作用。方法将日本大耳白兔18只随机分为芝麻素组、阿托伐他汀组和阳性对照组,基础饲料喂养。给药8周后测定各组0、5和8周末LDL-C水平;实验结束时取主动脉,常规HE染色及免疫组织化学染色,观察主动脉形态学变化和血管壁斑块组织的变化,定量分析血管VCAM-1表达强度变化。结果与阳性对照组相比,芝麻素组和阿托伐他汀组LDL-C水平显著降低（P〈0.01）,主动脉病理改变和血管壁斑块组织免疫着色明显减轻（P〈0.01）,主动脉壁VCAM-1表达水平分别下调27.59%和45.97%。与阿托伐他汀比较,芝麻素明显减轻主动脉中膜的厚度（P〈0.01）。结论芝麻素具有降低血脂、防治AS的作用。

Effects of sesamin on the formation of atheromatous plaque and the expression of VCAM-1 in atherosclerotic rabbit

Objective To observe the effects of sesamin on the formation of atheromatous plaque and expression of vascular cell adhesion molecule-1（VCAM-1） in atherosclerotic rabbit.Methods Eighteen Japanese white rabbits were randomly divided into sesamin group,atorvastatin group and positive control group.The treatment lasted for 8 weeks.Low-density lipoprotein cholesterol（LDL-C） was detected at 0,5 and 8 week after the administration in each group.By the end of experiment,all rabbits aortas were taken out to undergo pathological examination. The mRNA expression of VCAM-1 was detected by RT-PCR. Results The level of LDL-C in sesamin group and atorvastatin group was significantly decreased than that of positive control group （ P 〈 0.01 ）. Pathological changes of aorta and immunoehemical changes of vessel well plaque in the two treatment groups were significantly relieved than those in positive control group （P 〈0.01 ）. The downregulated expression rate of VCAM-1 of aortic wall in sesamin group and atorvastatin group was 27.59% and 45.97%, respectively. Conclusions Sesamin is benefit for the treatment of atheroselerosis as well as atorvastatin. The use of sesamin can improve atherosclerosis plaque and down-regulate VCAM-1 expression in aortic wall in addition hypolipemic.