HLA-DR基因与广西籍壮族IgA肾病相关性的研究

目的：探讨广西籍壮族IgA肾病（IgAN）与HLA—DR基因的相关性，并进行序列分析。方法：采用聚合酶链反应序列特异性引物法（PCR—SSP）对25例广西籍壮族IgAN患者及50名正常对照的HLA—DR4、HLA—DR12基因频率进行检测。并测定等位基因序列。结果：壮族IgAN组HLA—DR，的基因频率高于对照组（P〈0．05），HLA—DR4基因阳性组持续性镜下血尿伴蛋白尿的发生率高于阴性组（P〈0．05）。1例IgAN患者的HLA—DRB1＊04基因出现碱基对变异，149位A→G，临床表现为发作性肉眼血尿，病理类型为局灶节段性肾小球硬化。结论：①HLA—DR4基因可能是广西籍壮族IgAN的易感基因；②HLA—DR4基因与临床表现为持续性镜下血尿伴蛋白尿的广西籍壮族IgAN相关。

RELATIONSHIP OF HLA-DR GENE AND IgA NEPHROPATHY IN GUANGXI ZHUANG POPULATION

Objective:To evaluate relationship between HLA and IgA nephropathy,we study HLA-DR gene susceptibility to IgA nephropathy in Guangxi Zhuang population.Methods:HLA-DR_4 and HLA-DR_ 12 genes in 25 cases of IgA nephropathy patients and 50 normal controls were investigated by Polymerse Chain Reaction-Sequence Specific Primers(PCR-SSP) technique. Sequences of HLA-DR_4/HLA-DR_ 12 genes were analysed by direct sequencing of PCR products.Result:The frequency of HLA-DR_4 gene in IgA nephropathy patients was significantly higher than that in normal controls(P<0.05);and proteinuria is more frequent in HLA-DR_4-position patients than that in HLA-DR_4-negative patients (P<0.05).Conclusion:HLA-DR_4 gene may be susceptible allele in patients with IgA nephropathy in Guangxi Zhuang population,and HLA-DR_4 gene is associated with clinical presentation.