| 甲壳低聚糖对α-葡萄糖苷酶活性的影响及降血糖作用 |
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| 引用本文： | 曹朝晖,董晓英,方敏,李邦良.甲壳低聚糖对α-葡萄糖苷酶活性的影响及降血糖作用[J].中国生化药物杂志,2005,26(6):327-329. |
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| 作者姓名： | 曹朝晖 董晓英 方敏 李邦良 |
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| 作者单位： | 1. 南华大学,生物化学与分子生物学教研室,湖南,衡阳,421001
2. 长沙市卫生学校,湖南,长沙,410100 |
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| 基金项目： | 湖南省教育厅课题（No.4-02-JY-02C399）. |
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| 摘 要： | 目的探讨甲壳低聚糖对α-葡萄糖苷酶活性的影响及降糖效果。方法在体外建立标准酶反应体系,分别加入6%甲壳低聚糖50,100,150,200,250,300μl,用4-硝基酚-α-D呋喃葡萄糖苷法测定酶活性;再建立加有6%阿卡波糖200μl的酶反应体系,测定酶活性;以250 mg/(kg.d)剂量灌胃,观察其对实验性糖尿病大鼠空腹及餐后血糖的影响。结果甲壳低聚糖对α-葡萄糖苷酶具有明显抑制作用,抑制效果接近于阿卡波糖;甲壳低聚糖能显著降低糖尿病大鼠空腹及餐后血糖。结论甲壳低聚糖可能具有阿卡波糖样的降血糖作用。
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| 关 键 词： | 甲壳低聚糖 α-葡萄糖苷酶 抑制作用 |
| 文章编号： | 1005-1678（2005）06-0327-03 |
| 收稿时间： | 2004-11-13 |
| 修稿时间： | 2005-09-13 |
Effects of chitooligosaccharides on α-glucosidase and its lowering blood-glucose activity |
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| CAO Zhao-hui,DONG Xiao-ying,FANG Min,LI Bang-liang.Effects of chitooligosaccharides on α-glucosidase and its lowering blood-glucose activity[J].Chinese Journal of Biochemical Pharmaceutics,2005,26(6):327-329. |
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| Authors: | CAO Zhao-hui DONG Xiao-ying FANG Min LI Bang-liang |
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| Institution: | CAO Zhao-hui~1,DONG Xiao-ying~1,FANG Min~2,LI Bang-liang~1 |
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| Abstract: | PurposeTo study the effects of chitooligosaccharides on a-glucosidase activity and hypoglycemic activity in experimental type 2 diabetic rats.MethodsAn enzyme-inhibitor model was established to observe inhibition of α-glucosidase activity by chitooligosaccharides or acarbose,chitooligosaccharides or acarbose was given to mice at a dose of 250 mg/(kg·d)(ig,for 8 weeks),and the effect on glucose level of diabetic mice was observed.ResultsChitooligosaccharides could obviously inhibit α-glucosidase activity,and decrease the levels of fasting or pasting glucose.ConclusionThis finding suggests that the hypoglycemic effect of chitooligosaccharides is similar to that of acarbose. |
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| Keywords: | chitooligosaccharides α-glucosidase inhibition hypoglycemic effect |
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