| 原花青素抑制佐剂性关节炎大鼠PGE_2合成的机制 |
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| 引用本文： | 梁统,周克元,王旭光. 原花青素抑制佐剂性关节炎大鼠PGE_2合成的机制[J]. 沈阳药科大学学报, 2006, 23(11): 720-723 |
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| 作者姓名： | 梁统 周克元 王旭光 |
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| 作者单位： | 广东医学院,生物化学及分子生物学教研室,广东,湛江,524023 |
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| 基金项目： | 广东省教育厅自然科学基金;广东省社会科学基金 |
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| 摘 要： | 目的研究原花青素对佐剂性关节炎大鼠体内PGE2合成的抑制机理。方法建立大鼠佐剂性关节炎模型,EIA法测大鼠足爪炎症组织中PGE2的含量,RT PCR和Western blot分别检测大鼠腹腔巨噬细胞内COX 2 mRNA和蛋白的表达。结果原花青素显著降低佐剂性关节炎大鼠足爪炎症组织中的PGE2含量,下调大鼠腹腔巨噬细胞COX 2 mRNA和蛋白的表达。结论原花青素对佐剂性关节炎大鼠体内PGE2合成的抑制作用可能与其抑制COX 2 mRNA和蛋白表达有关。
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| 关 键 词： | 原花青素 佐剂性关节炎 前列腺素E2 |
| 文章编号： | 1006-2858(2006)11-0720-04 |
| 收稿时间： | 2006-02-08 |
| 修稿时间： | 2006-02-08 |
Inhibition mechanism of proanthocyanidins on the PGF2 product of rats suffered with adjuvant arthritis |
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| LIANG Tong,ZHOU Ke-yuan,WANG Xu-guang. Inhibition mechanism of proanthocyanidins on the PGF2 product of rats suffered with adjuvant arthritis[J]. Journal of Shenyang Pharmaceutical University, 2006, 23(11): 720-723 |
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| Authors: | LIANG Tong ZHOU Ke-yuan WANG Xu-guang |
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| Affiliation: | Department of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang 524023, China |
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| Abstract: | Objective To study the inhibition mechanism of proanthocyanidins on the PGE_2 product of rats with adjuvant arthritis(AA).Methods The adjuvant arthritis model of rats was induced by complete freunds adjuvant.The contents of PGE_2 in paws of rats were measured by EIA.The expression of COX-2 mRNA and protein in rat′s peritoneal macrophages was analysed by RT-PCR and Western blot.Results Proanthocyanidins could significantly suppress the contents of PGE_2 in edema paws and inhibit the expression of COX-2 mRNA and protein in peritoneal macrophages of rats suffered with adjuvant arthritis.Conclusions Inhibition mechanism of proanthocyanidins on the PGE_2 product of rats with adjuvant arthritis may be related to inhibiting the expression of COX-2 mRNA and protein. |
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| Keywords: | proanthocyanidins adjuvant arthritis prostaglandin E_2 |
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