A method of obtaining clone lines of cells

Summary A method of using microcapillaries filled with a small amount of nutrient medium is suggested for trapping and planting single cells in test-tubes. Trapping is done from a drop of cellular suspension on a slide, which is fixed under a microscope. Since the time of cloning is minimized cloning, cellular injury decreases, and the planting efficiency rises. The yield of colonies for A-1 strain was 63%, HeLa strain –50%, No. 630 strain –37% and Cave strain –10%. The number of planted single cells is unlimited. The use of test tubes simplified obtaining of clonal cell lines.(Presented by Active Member AMN SSSR N. N. Zhukov-Verezhnikov) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 57, No. 3, pp. 120–121, March, 1964.