电离辐射诱发小鼠胸腺淋巴瘤基因表达谱改变

目的探讨电离辐射诱发小鼠胸腺淋巴瘤基因表达谱的改变。方法1.75 Gy X射线每周1次,分4次照射BALB/c小鼠建立电离辐射诱发小鼠胸腺淋巴瘤模型,小鼠Mouse WG-6 v2.0全基因组表达谱微珠芯片筛选与正常小鼠胸腺差异表达基因,选取较重要的基因实时定量PCR验证。结果共筛选出3 063个差异表达基因,其中上调基因1 591个,下调基因1 472个,涉及KEGG数据库145个通路,BioCarta数据库76个通路及GenMAPP数据库218个通路;实时定量PCR结果显示,电离辐射诱发的小鼠胸腺淋巴瘤Bcl11b、Ikzf1基因mRNA表达水平均有下调趋势。结论电离辐射诱发小鼠胸腺淋巴瘤基因表达谱发生改变,涉及大量通路。

Differential gene expression profile induced by ionizing radiation In thymic lymphoma of mice

Objective To investigate the differences of gene expression induced by ionizing radiation in thymic lym-phoma of mice.Methods BALB/c mice were subjected to whole body irradiation with 1.75Gy X-ray once a week for 4 weeks to induce thymic lymphoma.The difference of gene expression in thymic cell of mice was detected by Mouse WG-6 v2.0 Expression BeadChip.The real-time PCR was used to identify important genes.Results The chip depicted 3 063 genes with differential gene expressions including 1 591 up-regulation and 1 472 down-regulation genes.The genes re-lated to 145 pathways of KEGG database,76 pathways of BioCarta database and 218 pathways of GenMAPP database.The results of real-time PCR showed that the mRNA expression level of Bcl11b and Ikzf1 genes tended to be down-regulated.Conclusion The ionizing radiation may induce differential gene expression profile in thymic lymphoma of mice, and the process may involve many pathways.