HPLC法同时测定消炎片中黄芩苷、黄芩素、汉黄芩素的含量

目的建立反相高效液相色谱法同时测定消炎片中黄芩苷、黄芩素、汉黄芩素的含量。方法采用Agilent Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm);以甲醇-体积分数0.4%磷酸溶液为流动相,梯度洗脱;检测波长为277 nm;流速为1.0 mL·min-1;进样量为5μL;柱温为30℃。结果黄芩苷、黄芩素、汉黄芩素的线性范围分别为8.01～128.2 mg·L-1(r=1.000 0,n=5)、5.36～85.76 mg·L-1(r=0.999 9,n=5)、5.34～85.44 mg·L-1(r=0.999 9,n=5);提取回收率分别为98.69%(RSD=0.6%,n=9)、98.29%(RSD=0.8%,n=9)、97.83%(RSD=1.0%,n=9)。结论该方法操作简便、快速、结果准确,可用于消炎片的质量控制。

Simultaneous determination of baicalin,baicalein and wogonin in Xiaoyan tablets by HPLC

Objective To establish RP-HPLC method for simultaneous determination of baicalin, baicalein and wogonin in Xiaoyan tablets (traditional Chinese medicines). Methods A column packed with Agilent Eclipse XDB-C₁₈(250 mm×4.6 mm, 5 μm)was used; the mobile phase was methanol-0.4%H₃PO₄ by gradient elution and the detection wavelength was set at 277 nm; the flow rate was 1.0 mL·min^-1, the injection volume was 5 μL, the column temperature was 30 ℃. Results The linear range of baicalin was from 13.82 to 125.6 mg·L^-1, r=0.999 8 (n=6); the linear range of baicalein was from 10.76 to 86.08 mg·L^-1, r=0.999 9 (n=6); the linear range of wogonin was from 8.35 to 66.80 mg·L^-1, r=0.999 9 (n=6). The average recovery of baicalin was 100.50%, RSD= 0.8%; the average recovery of baicalein was 99.76%, RSD= 1.5%; the average recovery of wogonin was 98.83%, RSD=1.2%. Conclusion　The method is found to be convenient, quick and accurate, and be used for the quality control of Xiaoyan tablets.