| HPLC-DAD同时测定柘树和构棘根与茎中4种黄酮类成分的含量 |
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| 引用本文： | 李波,王美,谭亚南,佟苗苗,翟延君.HPLC-DAD同时测定柘树和构棘根与茎中4种黄酮类成分的含量[J].中国中药杂志,2013,38(2):166-170. |
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| 作者姓名： | 李波 王美 谭亚南 佟苗苗 翟延君 |
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| 作者单位： | 辽宁中医药大学药学院,辽宁大连,116600 |
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| 基金项目： | 辽宁省教育厅课题(2008T121) |
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| 摘 要： | 目的:分析比较柘树与构棘根及茎中花旗松素、柑桔素、槲皮素、山柰酚的含量,为品种鉴定与质量评价提供科学参考.方法:采用高效液相二极管阵列检测法(HPLC-DAD),色谱柱为Agilent C18(4.6 mm×150 mm,5μm),流动相为甲醇(A)-0.1％磷酸溶液梯度洗脱(0 ～ 15 min,35％ ～50％ A;15 ～30 min,50％ ～65％A),检测波长290 nm(花旗松素、柑桔素)和365 nm(槲皮素、山柰酚),柱温30℃.结果:花旗松素、槲皮素的含量柘树根明显高于构棘根,柘树茎明显高于构棘茎,种间含量差异大；柑桔素、山柰酚的含量构棘根高于柘树根,柘树茎与构棘茎含量相当,种间含量差异小,同种药用部位间含量差异大.结论:4种成分的含量在柘树和构棘及其药用部位根和茎都存在一定的差异,在临床上不可混淆使用.
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| 关 键 词： | 柘树 构棘 HPLC-DAD |
| 收稿时间： | 2012/7/10 0:00:00 |
Simultaneous determination of four flavones in root and stem of Cudrania tricuspidata and C. cochinchinensis by HPLC-DAD |
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| LI Bo,WANG Mei,TAN Ya-nan,TONG Miao-miao and ZHAI Yan-jun.Simultaneous determination of four flavones in root and stem of Cudrania tricuspidata and C. cochinchinensis by HPLC-DAD[J].China Journal of Chinese Materia Medica,2013,38(2):166-170. |
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| Authors: | LI Bo WANG Mei TAN Ya-nan TONG Miao-miao and ZHAI Yan-jun |
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| Institution: | College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China;College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China;College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China;College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China;College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China |
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| Abstract: | |
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| Keywords: | Cudrania tricuspidata Cudrania cochinchinensis HPLC-DAD |
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