| 芫花致肝毒性部位筛选及其肝毒性部位UPLC指纹图谱研究 |
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| 引用本文： | 袁杨,耿璐璐,庄贺飞,孟夏,彭缨,毕开顺,陈晓辉.芫花致肝毒性部位筛选及其肝毒性部位UPLC指纹图谱研究[J].中国中药杂志,2013,38(1):70-74. |
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| 作者姓名： | 袁杨 耿璐璐 庄贺飞 孟夏 彭缨 毕开顺 陈晓辉 |
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| 作者单位： | 沈阳药科大学药学院,辽宁沈阳,110016 |
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| 基金项目： | 国家"重大新药创制"科技重大专项(2009ZX09301-012) |
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| 摘 要： | 目的:确定芫花药材致肝毒性部位,为全面反映并控制其毒性部位的质量建立该部位超高效液相色谱指纹图谱.方法:通过血清生化指标和组织病理学筛选芫花致肝毒性部位;并以采用ACQUITY UPLC BEH C18色谱柱(2.1 mm ×50mm,1.7μm),乙腈-0.05％磷酸溶液为流动相,梯度洗脱,210 nm为检测波长建立该部位UPLC指纹图谱.结果:确定了芫花致肝毒性部位为其乙醇提取物的氯仿萃取物;并建立了该部位的UPLC指纹图谱共有模式,标定了17个共有峰,指认了8个色谱峰,14批芫花药材致肝毒性部位的相似度为0.890～0.999.结论:芫花致肝毒性部位为其乙醇提取物的氯仿萃取物,所建立的UPLC指纹图谱,可为芫花临床安全用药提供依据.
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| 关 键 词： | 芫花 致肝毒性部位 指纹图谱 UPLC |
| 收稿时间： | 2012/7/27 0:00:00 |
Screening of hepatotoxicity fraction of Genkwa Flos and study on UPLC fingerprint of hepatotoxicity fraction |
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| YUAN Yang,GENG Lu-lu,ZHUANG He-fei,MENG Xi,PENG Ying,BI Kai-shun and CHEN Xiao-hui.Screening of hepatotoxicity fraction of Genkwa Flos and study on UPLC fingerprint of hepatotoxicity fraction[J].China Journal of Chinese Materia Medica,2013,38(1):70-74. |
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| Authors: | YUAN Yang GENG Lu-lu ZHUANG He-fei MENG Xi PENG Ying BI Kai-shun and CHEN Xiao-hui |
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| Institution: | School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China;School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China;School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China;School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China;School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China;School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China;School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China |
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| Abstract: | |
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| Keywords: | Genkwa Flos hepatotoxicity fraction fingerprint UPLC |
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