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| 清火栀麦片中化学成分的SPE-HPLC-ESI-MSn快速识别与鉴定 | |
| 引用本文： | 张加余,乔延江,高晓燕,张倩,马群,卢建秋.清火栀麦片中化学成分的SPE-HPLC-ESI-MSn快速识别与鉴定[J].中国中药杂志,2013,38(2):186-192. |
| 作者姓名： | 张加余 乔延江 高晓燕 张倩 马群 卢建秋 |
| 作者单位： | 1. 北京中医药大学中药学院,北京100102;北京中医药大学科研实验中心,北京100029 2. 北京中医药大学中药学院,北京,100102 3. 北京中医药大学科研实验中心,北京,100029 |
| 基金项目： | 中医药分析技术创新研究平台建设项目;北京市中药基础与新药研究重点实验室项目 |
| 摘 要： | 目的:建立一种固相萃取-高效液相色谱-电喷雾串联质谱(SPE-HPLC-ESI-MSn)分析方法,对清火栀麦片中主要的化学成分进行快速识别与鉴定.方法:根据HPLC-ESI-MSn负离子模式下的分子离子峰以及碎片离子获得化合物的结构信息,并通过与文献数据或对照品对照,确定化合物可能的结构.结果:通过对清火栀麦片及其组方药味进行比较分析,归属并鉴定了其中的39个化学成分,包括7个咖啡酰奎尼酸、7个环烯醚萜、6个二萜内酯、5个高异黄酮、13个甾体皂苷以及1个黄酮类成分.结论:该研究为鉴别清火栀麦片中的化学成分提供了一种简便、快速的方法. |
| 关 键 词： | 高效液相色谱-电喷雾串联质谱 固相萃取 清火栀麦片 化学成分鉴定 |
| 收稿时间： | 6/7/2012 12:00:00 AM |
Rapid recognition and identification of chemical constituents in Qinghuo Zhimai tablets by SPE-HPLC-ESI-MSn |
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| ZHANG Jia-yu,QIAO Yan-jiang,GAO Xiao-yan,ZHANG Qian,MA Qun and LU Jian-qiu.Rapid recognition and identification of chemical constituents in Qinghuo Zhimai tablets by SPE-HPLC-ESI-MSn[J].China Journal of Chinese Materia Medica,2013,38(2):186-192. | |
| Authors: | ZHANG Jia-yu QIAO Yan-jiang GAO Xiao-yan ZHANG Qian MA Qun and LU Jian-qiu |
| Institution: | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China;Center of Scientific Experiment, Beijing University of Chinese Medicine, Beijing 100029, China;School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China;Center of Scientific Experiment, Beijing University of Chinese Medicine, Beijing 100029, China;School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China;School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China;Center of Scientific Experiment, Beijing University of Chinese Medicine, Beijing 100029, China |
| Abstract: | Objective: To establish a method for analyzing solid phase extraction-high performance liquid chromatography-electrospray ionization tandem mass spectrometry (SPE-HPLC-ESI-MSn), in order to recognize and identify the main chemical constituents in Qinghuo Zhimai tablets. Method: The possible structures of the compounds were determined according to the structure information of compounds observed from molecular ion peaks and fragment ions in HPLC-ESI-MSn negative ion mode and by comparing with literature data or control samples. Result: Through the comparative analysis on Qinghuo Zhimai Tablets and components of its formula, 39 chemical constituents were identified, including 7 caffeoylquinic acids, 7 iridoids, 6 diterpenoid lactones, 5 homoisoflavonoids, 13 steroidal saponins and 1 flavone glycoside. Conclusion: This study provides a simple and rapid method for identifying chemical components in Qinghuo Zhimai tablets. |
| Keywords: | HPLC-ESI-MSn SPE Qinghuo Zhimai tablets identification of chemical constituents |
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