大豆总黄酮对大鼠肾虚型骨质疏松病的药效学研究

目的:观察大豆总黄酮对大鼠骨质疏松的治疗作用.方法:采用12月龄雌性大鼠,切除卵巢60 d以后,以大豆总黄酮250,125,62.5 mg·kg-13个剂量连续灌胃(ig)90 d.测血清碱性磷酸酶(ALP)、钙离子(Ca2+)、磷离子(p3-)、钠离子(Na+)、雌二醇(E2)含量;原子吸收法测定骨Ca2,p3-,Na+,Mg2含量;骨扫描法测定胫骨骨密度.结果:以大豆总黄酮250,125 mg·kg -1可以增加骨质疏松大鼠血Ca+2,E2,对血中p3-,Na+离子,ALP含量无明显影响;提高骨Ca2+含量,对骨p3-含量具有一定的升高趋势,对骨Na+,Mg2+无明显影响;增加骨密度和骨小梁面积;减少骨吸收,促进骨形成.结论:大豆总黄酮具有治疗肾虚大鼠骨质疏松作用.

Effects of Soybean Flavone on Osteoporosis in Rats with Deficiency of the Kidney

Objective: To observe effects of soybean flavone on osteoporosis in rats with deficiency of the kidney. Method: Female rats born 12 monthes were executed orariectomy to establish osteoporosis model. After 60 days, total flavonoids of soybean, with dose of 250, 125, 62.5 mg·kg^-1,was ig given for 90 days. The content of ALP, Ca²⁺,P^3-, Na⁺ estradiol(E₂) in serum, the content of Ca²⁺,P^3-,Na⁺,Mg²⁺in bones was measured,respectively. The bone density of tibia was messured. Result: The level of Ca²⁺, E₂ of in serim was increased in total flavonoids 250, 125 mg·kg^-1 groups, but had no obvious effect on the content of P^3-,Na⁺,ALP in blood; The content of Ca²⁺ in bones was increased, but had no obvious influence on Na⁺,Mg²⁺ in bones, meanwhile the bone density wa increasedd. Conclusion: Soybean flavone has effects on osteoporosis in rats with deficiency of the kidney