冰糖草挥发油化学成分的GC-MS分析

目的:研究广西产冰糖草挥发油的化学组成及相对含量.方法:采用水蒸气蒸馏法提取黄杞叶中的挥发油,并通过气相色谱-质谱(GC-MS)联用仪对其化学成分进行分析和鉴定,用色谱峰面积归一化法计算各组分相对百分含量.结果:从冰糖草挥发油中共分离出49个组分,鉴定了其中30个化合物,占总量的93.97％,主要成分为植酮(2-Pentadecanone,6,10,14-trimethyl-,19.75％)、石竹烯(caryophyllene,15.33％)、α-石竹烯(alpha.-caryophyllene,10.14％)、1S-(1,3a,3b,6a,6b) -十氢-3a-甲基-6-亚甲基-1-异丙基-环丁烷-[1,2,3,4]并二环戊烯、(cyclobuta[1,2:3,4]dicyclopentene,decahydro-3 a-methyl-6-methylene-1 -(1 -methylethyl) -,[ 1S-(1.alpha.,3a.alpha.,3b.beta.,6a.beta.,6b.alpha.)],6.53％)、氧化石竹烯(Caryophyllene oxide,4.90％)、表双环倍半水芹烯[(+)-Epi-bicyclosesquiphellandrene,4.69％]、芳姜黄酮(Ar-tumerone,4.57％)、十七烷( heptadecane,4.13％)、肉豆蔻醛(tetradecanal,2.33％)、邻苯二甲酸异丁基十一烷酯(phthalic acid,isobutyl undecyl ester,2.11％)等.结论:广西产冰糖草挥发油中含脂肪酸、酯类、醇类、醛类、烃类等多种化学成分;分析结果可为冰糖草的质量控制提供依据,并为提高冰糖草的进一步开发利用提供了科学依据.

GC-MS Analysis of Chemical Components of Volatile Oil from Sweet Broomwort Herb

Objective: To ananlyze the chemical constituents of the volatile oil from Sweet Broomwort Herb in Guangxi and determine their contents. Method: The volatile oil was extracted from Sweet Broomwort Herb by steam distillation, the amount of the components from the volatile oil was separated and identified by GC-MS, and the relative content of each component was calculated by area normalization method. Result: Fourty nine compounds were separated by GC and 30 of them were identified, which accounted for 93.97% of volatile oil. The major components were 2-pentadecanone,6,10,14-trimethyl-(19.75%),caryophyllene(15.33%),alpha.-caryophyllene(10.14%),(cyclobuta[1,2:3,4]dicyclopentene,decahydro-3a-methyl-6-methylene-1-(1-methylethyl)-,[1S-(1.alpha.,3a.alpha.,3b.beta.,6a.beta.,6b.alpha.)](6.53%),caryophyllene oxide (4.90%), (+)-epi-bicyclosesquiphellandrene (4.69%), Ar-tumerone (4.57%), heptadecane (4.13%), tetradecanal (2.33%), phthalic acid, isobutyl undecyl ester (2.11% ) et al. Conclusion: The volatile oils mainly consisted of fatty acid, esters, alcohols, aldehydes, hydrocarbons; the analytic results can provided evidence for quality control and further exploitation of Sweet Broomwort Herb.