远志水提取物对小鼠学习记忆及血液学指标的影响

目的:观察远志水提取物对小鼠学习记忆及力竭运动后血液学指标的影响.方法:将84只小鼠随机分为学习记忆组和运动组.学习记忆组小鼠48只,又分为对照组和远志高、中、低剂量组,分别给予远志水提取物13.00,6.50,3.25 g·kg-1 ·d-1灌胃21 d后,利用Morris水迷宫观察小鼠的学习记忆能力,同时测定脑组织中超氧化物歧化酶(SOD)、丙二醛(MDA).运动组小鼠36只,再分为对照组,游泳组和远志组.除对照组外,游泳组和远志组小鼠进行3周的游泳训练,最后一次力竭游泳后心脏采血,测定红细胞计数(RBC)和血红蛋白(Hb).结果:学习记忆组小鼠灌胃远志水提取物后,远志水提取物中剂量组和高剂量组找到站台的潜伏期明显缩短(P＜0.05),穿越站台次数明显增加(P＜0.05).中剂量组和高剂量组小鼠脑组织SOD活性明显增高,MDA含量明显减少,与对照组相比有统计学意义(p＜0.05).低剂量组各指标与高剂量组比较均有统计学意义(P＜0.05).运动组中,灌胃远志后小鼠负重游泳力竭时间与对照组相比明显延长(P＜0.01).血液学指标显示,对照组小鼠RBC和Hb均低于没有进行游泳训练的空白对照组(P＜0.05).而远志组小鼠RBC和Hb与空白对照组比较无明显差异,与对照组比较明显增加(P＜0.05).结论:远志水提取物可增强小鼠的学习记忆能力,增加脑组织SOD活性,降低MDA含量;远志水提取物可增强小鼠的运动能力,并能有效抑制大强度运动引起的RBC和Hb改变.

Effects of Water Extract from Polygalae Radix on Learning and Memory Abilities and Index of Hematology in Mice

Objective: To observe the effects of water extract from Polygalae Radix on learning and memory abilities and index of hematology in mice. Method: Eighty four Kunming mice were randomized for learning and memory test and motion test. In learning and memory test forty eight mice were randomly divided into control group, low-dose, middle-dose and high-dose group of extracts from Polygalae Radix. After continuous intragastric administration of twenty one days, the ability of learning and memory was observed and the activity of superoxide dismutases (SOD) and the content of maleic dialdehyde (MDA) was determined in brain. In motion group thirty six mice were randomly divided into control group, swimming group and Polygalae Radix group. Except control group, swimming training was executed in the other groups for three weeks. After the last weight-loaded swimming, the RBC and Hb were determined. Result: Compared with control group the latency period of the mice which were given the middle-dose and high-dose extracts from Polygalae Radix were significantly shorten(P<0.05),and the times of passing through the flatform were significantly increased(P<0.05).As well as the activity of SOD significantly increased and the content of MDA significantly decreased compared with control group(P<0.05). In motiontest, the weight-loaded swimming time of the mice which were given extracts from Polygalae Radix was signicantly extended compared with control group(P<0.05). Compared with blank group the red blood corpuscle and hemoglobin level in control group were significantly depressed(P<0.05).However, the red blood corpuscle and hemoglobin level in Polygalae Radix group were increased compared with control group(P<0.05)and similar with blank group. Conclusion: The water extract from Polygalae Radix can enhance the ability of learning and memory in mice, raise the activity of SOD and depress the content of MDA. The water extract from Polygalae Radix can increase the ability of motor and suppressed the change of red blood corpuscle and hemoglobin after strenuous exercise.