槲皮素对H2O2致内皮细胞损伤的保护作用

目的:探讨槲皮素对血管内皮细胞损伤的保护作用及机制.方法:采用体外培养的人脐静脉内皮细胞株ECV304,观察内皮细胞加入槲皮素8,4,2 mg·L-1培养24 h后,再加入H2O2培养18h后,造成血管内皮细胞损伤模型.用流式细胞仪测定内皮细胞死亡率,用ELISA法检测内皮细胞培养液血栓调节蛋白(TM)的含量;荧光分光光度法测定内皮细胞培养液中乳酸脱氢酶(LDH)的活性.结果:与H2O2损伤后的模型组相比,槲皮素可以减低细胞死亡率,模型组细胞死亡率为(28.36±0.10)％,槲皮素高、中、低剂量分别为(8.34±0.01)％,(16.58±0.04)％,(10.12±0.02)％],可使培养液中TM蛋白含量减少,模型组为(58.5±18.8)μg·L-1,槲皮素高、中、低剂量为(32.7±9.7),(27.8±1.9),(32.1±8.6) μg·L-1]和LDH活性减少模型组为(1 931.9±159.5) U·L-1,槲皮素高、中、低剂量为(1.092±126.2),(1 159.6±274.2),(1 223.5±120.5)U·L-1].结论:槲皮素能明显保护过氧化氢对内皮细胞的损伤,其保护作用与抗脂质过氧化、保护细胞的完整性有关.

Protective Effect of Quercetin on Endothelial Cell Injured by H2O2

Objective: To evaluate the effects of quercetin on the injured vascular endothelial cells and its mechanism. Method: Endothelial cell culture was in vitro. The endothelial cell injury model was established by cultured with H₂O₂ for 18 h, Quercetin at dose of 8,4,2 mg·L^-1 was cultured with the cells before the model establishment. Flow cytometry was used for determining endothelial cell death rate. Endothelial cell was detected by ELISA for medium thrombomodulin(TM) content; spectrophotometric determination was used for the culture medium lactate dehydrogenase(LDH) activity. Result: Compared to the model group, quercetin can lower endothelial cell death rate,reduce the content of TM and activity of LDH in the culture medium. Conclusion: Quercetin can obviously protect endothelial cells, and its protective effect is related to against lipid peroxidation, protection of the cell membrane.