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| 体外循环手术对红细胞CD35、CD59及T淋巴细胞免疫的影响 | |
| 引用本文： | 胡金川,田亚平,李佳春,董培青,万彩红,谷峰,骆荩,高艳红,江朝光.体外循环手术对红细胞CD35、CD59及T淋巴细胞免疫的影响[J].中国体外循环杂志,2008,6(4):210-212. |
| 作者姓名： | 胡金川 田亚平 李佳春 董培青 万彩红 谷峰 骆荩 高艳红 江朝光 |
| 作者单位： | 1. 中国人民解放军总医院,生化科,北京,100853 2. 中国人民解放军总医院,心血管外科,北京,100853 3. 北京安贞医院体外循环科,北京,100029 |
| 基金项目： | 解放军重点项目 |
| 摘 要： | 目的探讨体外循环（extracorporeal circulation，ECC）手术对红细胞CD35、CD59及T淋巴细胞免疫功能的影响。方法选择ECC手术病例18例，于转机前、体温最低点、转机末和术后1d,3d,7d晨采集患者静脉血，测定红细胞CD35、CD59，以及T淋巴细胞CD3、CD4和CD8。结果ECC前至ECC末，CD35^＋红细胞（CD35^＋-E）百分率、CD59几何平均荧光强度比值（CD59-GMFIR）和CD35-GMFIR均逐渐降低，前二者在术后1d至7d逐渐回升，而CD35-GMFIR未见明显阪复趋势。ECC末、术后1d时CD35^＋-E百分率和CD35-GMHR，以及体温最低点、ECC末和术后1d时CD59-GMFIR均显著低于ECC前（P〈0．01或P〈0．05）。至术后7d时，CD35^＋-E百分率、CD35-GMFIR和CD59-GMHR仍束恢复至ECC前水平。从ECC前至术后7d，CD3^＋T淋巴细胞（CD3^＋-T）百分率、CD3^＋CD4^＋-T百分率和CD4^＋-T／CD8’-T比值均呈现先降低后升高的趋势，体温最低点、ECC末、术后1d和术后3d的CD3^＋-T百分率、CD3^＋CD4^＋-T百分率均显著低于ECC前（P〈0．01或P〈0．05），至术后7d恢复至接近于ECC前水平，而CD4^＋-T／CD8^＋-T比值则恢复至超过ECC前水平。结论ECC手术使红细胞CD35、CD59及T淋巴细胞免疫功能下降，后者于术后7d恢复．前者恢复期长于后者。 |
| 关 键 词： | 体外循环 红细胞免疫 CD35抗原 CD59抗原 T淋巴细胞 |
Effects of Extracorporeal Circulation Operation on CD35 and CD59 of Erythrocytes and T Lymphocytes Immune |
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| HU Jin-chuan,TIAN Ya-ping,LI Jia-chun,DONG Pei-qing,WAN Cai-hong,GU Feng,LUO Jin,GAO Yan-hong,JIANG Chao-guang.Effects of Extracorporeal Circulation Operation on CD35 and CD59 of Erythrocytes and T Lymphocytes Immune[J].Chinese Journal of Extracorporeal Circulation,2008,6(4):210-212. | |
| Authors: | HU Jin-chuan TIAN Ya-ping LI Jia-chun DONG Pei-qing WAN Cai-hong GU Feng LUO Jin GAO Yan-hong JIANG Chao-guang |
| Institution: | HU Jin-chuan1,TIAN Ya-ping1,LI Jia-chun2,DONG Pei-qing3,WAN Cai-hong3,GU Feng1,LUO Jin2,GAO Yan-hong1,JIANG Chao-guang1(1.Department of Clinical Biochemistry,2.Department of Cardiovascular Surgery,PLA General Hospital,Beijing 100853,3.Department of Cardiopulmonary Bypass,Anzhen Hospital,Beijing 100029,China) |
| Abstract: | OBJECTIVE To study the effects of extracorporeal circulation(ECC) operation on CD35 and CD59 of erythrocytes,and T lymphocytes immune.METHODS Eighteen cases of heart operation under ECC were included in this study.Venous blood samples were collected at pre-ECC,time of minimum body temperature,end of ECC,and 1 day,3 days,7 days after operation,then CD35 and CD59 on erythrocytes,and CD3,CD4 and CD8 on T lymphocytes were measured by flow cytometry immediately.RESULTS The CD35 positive erythrocytes(CD35+-E) per... |
| Keywords: | Extracorporeal circulation Red-cell immune CD35 antigen CD59 antigen T lymphocytes |
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