+GZ暴露对大鼠脑热休克蛋白-70表达水平的影响

目的 探讨＋Ｇｚ暴露条件下大鼠脑中热休克蛋白－７０（ＨＳＰ７０）的变化及其在＋Ｇｚ所致脑损伤中的作用。方法 １００只大鼠，随机分为４胡，每组２５只，一组作为空白对照，其余三组分别进行＋２Ｇｚ，＋６Ｇｚ和１０Ｇｚ暴露，峰值作用时间３ｍｉｎ，加速度增长率１Ｇ／ｓ然后，分别于暴露后的６ｈ、１ｄ、２ｄ、４ｄ和６ｄ麻醉大鼠，心脏灌注后迅速取脑组织，用Ｗｅｓｔ ｂｌｏｔ法比较ＨＳＰ７０的变化情况。结果 ＋Ｇｚ暴露后６ｈＨＳＰ７０开始升高，１ｄ达到高峰，至６ｄ时基本恢复正常。不同＋Ｇｚ暴露之间进行比较可以看出，＋６ＧＧｚ组ＨＳＰ７０表达最高，＋１０Ｇｚ最少。结论 ＋Ｇｚ暴露对大鼠脑ＨＳＰ７０表达水平有显著的影响。

Effect of G_Z exposures on expression of heat shock protein 70 in the rat brain

Objective To explore the effect of G Z exposures on expression of heat shock protein 70(HSP70) in the rat brain. Methods One hundred SD rats were randomly divided into 4 groups: 1 control group, and 3 experimental groups, which underwent 2 G Z, 6 G Z, 10 G Z exposures for 3 min respectively. The expression levels of HSP70 in rat brain were measured by West blot 6 h, 1 d, 2 d, 4 d and 6 d after G Z exposures. Results As compared with control, the expression levels of HSP70 began to raise 6 h after G Z exposure, up to the peak value 1 d later, and returned to normal after 6 d. As to the degree of change of HSP70 expression following various G Z exposures, the most obvious elevation was observed after 6 G exposure, and the least after 10 G exposure. Conclusion Increase of HSP70 expression in rat brain can be induced by G Z exposures. It may play a role in protecting the brain from injury following G Z exposures.