中药丹参注射液对兔脊髓损伤的影响

目的 :探讨中药丹参注射液对免脊髓损伤的保护作用。方法 :成年新西兰大白兔按Allen’s方法略作改进 ,致T12～T1损伤 ,打击力为 15 0g·cm- 1 ,造成脊髓全瘫模型 ,分别给予甘露醇、N 硝基精氨酸甲酯 (L NAME)和丹参注射液治疗 ,于术前、术后 4h ,6h取静脉血测定丙二醛 (MDA) ,超氧化物歧化酶 (SOD) ,一氧化氮 (NO) ;脊髓行常规组织切片和iNOS免疫组化染色。结果 :①损伤组血中MDA↑ ,SOD↓ ,NO↑ ,而各治疗组上述指标均有改善 ,各组各指标比较差异有显著性 (P <0 .0 1) ;②组织学检查损伤组有神经细胞变性 ,坏死 ,各治疗组程度略轻 ;iNOS在各组中均有表达 ,丹参组iNOS表达最明显 ,而L NAME组则较少表达。结论 :早期使用丹参注射液对脊髓损伤有一定保护作用

Protective effect of Dansheng injection on experimental rabbits'' spinal cord injury

Objective To investigate the protective effect of Dansheng injection on experimental rabbits’ in spinal cord injury. Methods Thirty two New Zealand rabbits were impacted between T12 T1 with a force of 150 g·cm -1 (10g×15cm). The rabbits were randomly divided into the injury group, monitor group,L NAME group and Dansheng group. The blood was obtained at 0 h, 4 h, 6 h to seperate the serum at the end the injured spinal cord. Cord tissues were obtained for histological examination. Methyl di aldehyde (MDA), superoxide dismutase (SOD) and nitro oxide (NO) from the serum and spinal tissues homogenated were tested. Results MDA and NO increased in the injury group compared with the others, but SOD significantly decreased in the injury group compared with the others. Histological examination showed that neurons were degenerated and necrosed. The injury group displayed serious changes while the Dansheng group less changes. iNOS expressed in all the groups. Conclusion Dansheng injection has some protective effect on spinal cord injury in the early stage.