A gel casting technique allowing rapid and sensitive quantitation of antibodies by diffusion-in-gel enzyme-linked immunosorbent assay |
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| Authors: | H Nygren M Stenberg |
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| Affiliation: | 1. Department of Histology, University of Göteborg, Göteborg, Sweden;2. Research Laboratory of Electronics, Chalmers University of Technology, Göteborg, Sweden |
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| Abstract: | A technique is described which allows rapid and sensitive quantitation of antibodies by diffusion towards an antigen-coated surface through a gel of wedge-shaped profile. A sensitivity of 0.16 micrograms antibody per ml solution was obtained after 45 min incubation with stirring at 22 degrees C and 0.08 micrograms/ml could be detected after 90 min incubation with stirring or with incubation at 37 degrees C. |
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| Keywords: | diffusion-in-gel ELISA immunodiffusion technique |
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