肝纤维化与肝硬化患者肝内CXCL13和CXCL16的表达变化

目的　观察正常人和肝纤维化与肝硬化病人肝组织中趋化因子CXCL13和CXCL16的表达变化。方法　用ELISA方法分别检测9例正常人和10例肝纤维化、11例肝硬化病人肝组织中的CXCL13和CXCL16含量。结果　正常对照组、肝纤维化组、肝硬化组的CXCL13浓度分别为(2.34±2.05)、(6.04±2.97)和(12.10±10.38) ng/g；肝硬化组的浓度显著高于正常对照组和肝纤维化组，正常对照组与肝纤维化组的CXCL13浓度差异无统计学意义。正常对照组、肝纤维化组、肝硬化组的CXCL16浓度分别为(1.32±0.91)、(3.34±1.81)和(3.49±1.90) ng/g；肝硬化组和肝纤维化组浓度均显著高于正常对照组浓度，肝硬化组和肝纤维化组的CXCL16浓度差异无统计学意义。结论　肝脏发生纤维化损伤时，肝脏表达CXCL13和CXCL16的数量都显著增多，但二者的变化规律不同。肝内CXCL13水平在肝纤维化阶段开始升高，至肝硬化阶段升高到显著水平。肝内CXCL16水平在肝纤维化阶段就已经升高到显著水平，至肝硬化阶段仍维持在高水平状态。

Hepatic expressions of CXCL13 and CXCL16 in patients with hepatic fibrosis and cirrhosis and in normal controls

Objective To investigate the level of chemotactic factor (CXCL13 and CXCL16) in liver tissue in healthy human and patients with hepatic fibrosis and cirrhosis. Methods Hepatic tissues were obtained in surgery from 9 normal persons, 10 patients with fibrotic liver, 11 patients with cirrhosis. The content of CXCL13 and CXCL16 in hepatic tissue was assayed by ELISA. Results The concentration of CXCL13 was (2.34±2.05) ng/g in normal liver, (6.04±2.97) ng/g in fibrotic liver and (12.10±10.38) ng/g in cirrhosis. The level of CXCL13 in sclerous liver was remarkably higher than those in normal and fibrotic liver, but there was no significant difference between normal liver and fibrotic liver. The concentration of CXCL16 was (1.32±0.91) ng/g in normal liver, (3.34±1.81) ng/g in fibrotic liver and (3.49±1.90) ng/g in cirrhosis. The level of CXCL16 in fibrotic liver and sclerous liver were remarkably higher than those in normal liver, but there was no significant difference between fibrotic liver and sclerous liver. Conclusion With the aggravation of hepatic fibrous degeneration, intrahepatic levels of CXCL13 and CXCL16 were significantly increased, but the laws of chang were different between them. Intrahepatic level of CXCL13 began to rise in the stage of liver fibrosis and had a significant increase in cirrhosis stage. Intrahepatic level of CXCL16 had already increased to significant level in the stage of liver fibrosis, and it remianed in a high state in liver cirrhosis stage.