| 流式细胞仪检测人白细胞抗原-B_(27)的影响因素 |
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| 引用本文： | 曹鲁宁,张玲珍,仲人前,孔宪涛. 流式细胞仪检测人白细胞抗原-B_(27)的影响因素[J]. 上海医学检验杂志, 2007, 0(3) |
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| 作者姓名： | 曹鲁宁 张玲珍 仲人前 孔宪涛 |
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| 作者单位： | 第二军医大学附属长征医院实验诊断科 上海200003 |
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| 摘 要： | 目的获得外周血淋巴细胞人白细胞抗原(HLA)-B27检测准确可靠的结果。方法用双抗体双色免疫荧光标记,流式细胞仪分析,对标本放置不同时间、溶血后洗涤前后的结果进行比较。结果标本放置不同时间可影响检测结果,洗涤可显著增加HLA-B27阳性细胞百分比和细胞表面平均荧光强度。结论制备好的标本应在6 h内检测,溶血后标本宜洗涤后测定,以提高检测的准确性。
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| 关 键 词： | 人白细胞抗原-B27 流式细胞仪 影响因素 |
The influencing factors of detecting human leucocyle antigen-B_(27) by flow cytometry |
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| CAO Luning,ZHANG Lingzhen,ZHONG Renqian,KONG Xiantao.. The influencing factors of detecting human leucocyle antigen-B_(27) by flow cytometry[J]. Shanghai Journal of Medical Laboratory Sciences, 2007, 0(3) |
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| Authors: | CAO Luning ZHANG Lingzhen ZHONG Renqian KONG Xiantao. |
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| Abstract: | Objective To search an accurate way to determine human leucocyte antigen(HLA)B_(27) of peripheral blood cells. Methods By means of bicolour fluorescence and flow cytometry(FCM) analysis,the results were compared during different lay-out time,hemolysis time and washing time. Results The results were influenced during different lay-out time.Besides,the percentage of HLA-B_(27) positive cells and the mean fluorescence intensity were significantly enhanced by washing. Conclusions Samples should be tested in six hours after preparation and washing the hemolysis samples could improve the accuracy of detection. |
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| Keywords: | Human leucocyte antigen-B_(27) Flow cytometry Influencing facfor |
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