| 人骨髓间充质干细胞的分离培养和向神经细胞分化的研究 |
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| 引用本文： | 金钧,单立冬,惠国桢,苗宗宁,陆华.人骨髓间充质干细胞的分离培养和向神经细胞分化的研究[J].实用临床医药杂志,2003,7(3):197-198,201. |
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| 作者姓名： | 金钧 单立冬 惠国桢 苗宗宁 陆华 |
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| 作者单位： | 1. 苏州大学附属第一医院,江苏,苏州,215007
2. 江苏省无锡市第三人民医院,江苏,无锡,214001 |
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| 基金项目： | 国家自然基金资助项目(3 0 2 713 2 5 )，江苏省自然基金资助项目(BK2 0 0 1170 ) |
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| 摘 要： | 目的 分离骨髓后培养人骨髓间充质干细胞 (hMSCs) ,进行体外传代扩增 ,并探索体外诱导分化为神经元样细胞的方法。方法 抽取人骨髓血 ,percoll paque液 (1 0 73g/ml)分离 ,DMEM/2 0 %FCS培养传代 ,至第 3～ 5代时加入巯基乙醇 (BME) ,观察hMSCs分化为神经元样细胞的形态学变化。结果 hMSCs在体外可以实现数目扩增 ,在特定诱导剂的作用下向神经元样细胞分化。结论 采用少量骨髓样本 ,经培养获得充足的细胞量 ,满足定向诱导和细胞移植的需要。
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| 关 键 词： | 骨髓间充质干细胞 诱导分化 巯基乙醇 神经细胞 |
| 文章编号： | 1672-2353(2003)03-0197-03 |
THE ISOLATION AND CULTURE OF HUMAN MESENCHYMAL STEM CELLS AND DIFFERENTIATION INTO NEURONS |
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| JIN Jun ,SHAN Li-dong ,HUI Guo-zhen ,MIAO Zong-nin ,LU Hua.THE ISOLATION AND CULTURE OF HUMAN MESENCHYMAL STEM CELLS AND DIFFERENTIATION INTO NEURONS[J].Journal of Clinical Medicine in Practice,2003,7(3):197-198,201. |
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| Authors: | JIN Jun SHAN Li-dong HUI Guo-zhen MIAO Zong-nin LU Hua |
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| Institution: | JIN Jun 1,SHAN Li-dong 1,HUI Guo-zhen 1,MIAO Zong-nin 2,LU Hua 2 |
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| Abstract: | Objective: To investigate the methods of hMSCs differentiating into neurons, while human bone marrow were isolated and cultured in vitro. Methods: hBMCs were separated with Percoll-Paque regent, and MSCs were cultured and expanded in vitro. Then at Passage3 hBMSCs were induced with β-mercaptoethanol (BME). And neuron morphology was we observed. Results: hMSCs can expand in vitro and BME can induce cells into neuron morphology. Conclusion: A little bone marron can obtain sufficiently cells for induction and cells transplantation. |
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| Keywords: | mesenchymal stem cells(MSCs) β?mercaptoethanol(BME) induction and differentiation neuron |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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